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Title: Spatio-temporal radiation biology with conventionally or laser-accelerated particles for ELIMED

The aim of this study is to investigate the behavior of radio-resistant human malignant cells, thus enabling better understanding of radiobiological effects of ions in such a case. Radiation sources such as accelerated continuous ion beams and laser technology-based ultra short radiation sources with energy of around 10 MeV will be used. The HTB140 melanoma cells are chosen since it has been shown that they represent the limit case of cellular radio-resistance among the studied tumor cell lines. These cells are particularly interesting as they provide data on the very edge of inactivation capacity of each beam line that is tested. After exposing the cell monolayers to continuous radiations of low (γ-rays) and high (protons) linear energy transfer, the kinetics of disappearance of the phosphorylated histone H2AX (γ-H2AX) foci per cell will be determined. The same procedure will be performed with the pulsed high dose rate protons. Detection and quantification of γ-H2AX foci will be performed by immunohistochemical 3D time-dependent imaging analyses using laser scanning confocal microscopy. Immunoblotting will enable the follow-up of the relation between γ-H2AX and cell cycle arrest via the p53/p21 pathway. In such a way the spatio-temporal changes on sub-cellular level will be visualized, quantified andmore » compared. These results will show whether there is a difference in the effects on cells between continuous and pulsed irradiation mode. Therefore, they will contribute to the data base that might promote pulsed sources for medical treatments of malignant growths.« less
Authors:
; ; ;  [1] ; ; ;  [2]
  1. Vinča Institute of Nuclear Sciences, University of Belgrade, P.O. Box 522, 11001 Belgrade (Serbia)
  2. Istituto Nazionale di Fisica Nucleare, Laboratori Nazionali del Sud, via S. Sofia 62, Catania (Italy)
Publication Date:
OSTI Identifier:
22218272
Resource Type:
Journal Article
Resource Relation:
Journal Name: AIP Conference Proceedings; Journal Volume: 1546; Journal Issue: 1; Conference: 2. ELIMED workshop and panel, Catania (Italy), 18-19 Oct 2012; Other Information: (c) 2013 AIP Publishing LLC; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; BIOCHEMISTRY; BIOLOGICAL RADIATION EFFECTS; CELL CYCLE; COMPARATIVE EVALUATIONS; DOSE RATES; DRUGS; GAMMA RADIATION; HUMAN POPULATIONS; INACTIVATION; MELANOMAS; MEV RANGE; OPTICAL MICROSCOPY; PROTON BEAMS; PROTON DETECTION; PULSED IRRADIATION; RADIOBIOLOGY; RADIOTHERAPY; TIME DEPENDENCE; TUMOR CELLS