Expression of paired-like homeodomain transcription factor 2c (PITX2c) in epidermal keratinocytes
- Department of Dermatology, School of Medicine, Chungnam National University, Daejeon, 301-747 (Korea, Republic of)
- Department of Dermatology, The First Affiliated Hospital, Guangxi Traditional Chinese Medical University, Guangxi, Nanning, 530023 (China)
- Department of Anatomy, School of Medicine, Chungnam National University, Daejeon, 301-747 (Korea, Republic of)
- Department of Dermatology, School of Medicine, Gyeongsang National University, Jinju, 660-702 (Korea, Republic of)
- Department of Dermatology, Chonnam National University Medical School, Gwangju, 501-757 (Korea, Republic of)
Paired-like homeodomain transcription factor 2 (PITX2) has been implicated as one of the genes responsible for Rieger syndrome. It has been also shown to play a central role during development. In this study, we investigated the functional role of PITX2 in keratinocyte differentiation. RT-PCR analysis showed that PITX2c isoform was predominantly expressed in a differentiation-dependent manner. Consistent with, immunohistochemical staining showed that PITX2 expression was increased in the upper layer of epidermis. When PITX2c was overexpressed in cultured keratinocytes by a recombinant adenovirus, the differentiation markers such as involucrin and loricrin were significantly increased at both mRNA and protein levels. In addition, PITX2c overexpression led to the decrease of cell growth, concomitantly with the upregulation of cell cycle-related genes p21. To investigate the effect of PITX2c in vivo, we microinjected PITX2c expression vector into zebrafish embryo. Interestingly, overexpression of PITX2c in zebrafish embryo led to the formation of horn-like structure and thickening of epidermis, together with the increase of keratin 8 (K8) expression. These results suggest that PITX2c has a role in proliferation and differentiation of epidermal keratinocytes.
- OSTI ID:
- 22209928
- Journal Information:
- Experimental Cell Research, Vol. 316, Issue 19; Other Information: Copyright (c) 2010 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); ISSN 0014-4827
- Country of Publication:
- United States
- Language:
- English
Similar Records
Keratin Promotes Differentiation of Keratinocytes Seeded on Collagen/Keratin Hydrogels
A novel mechanism of filaggrin induction and sunburn prevention by β-damascenone in Skh-1 mice