Increased cellular apoptosis susceptibility (CSE1L/CAS) protein expression promotes protrusion extension and enhances migration of MCF-7 breast cancer cells
- Section of Hematology-Oncology, Taipei Medical University and Hospital, Taipei, Taiwan (China)
- Graduate Institute of Medical Science, Taipei Medical University and Hospital, Taipei, Taiwan (China)
- Institute of Cellular and Organismic Biology, Academia Sinica, Taipei, Taiwan (China)
- Institute of Biomedical Materials and Engineering and Center of Excellence for Cancer Research, Taipei Medical University and Hospital, Taipei, Taiwan (China)
- School of Public Health, Taipei Medical University and Hospital, Taipei, Taiwan (China)
- Department of Surgery, Tungs' Taichung MetroHarbor Hospital, Taichung, Taiwan (China)
- Breast Center, Taipei Medical University and Hospital, Taipei, Taiwan (China)
- Cancer Center, Taipei Medical University and Hospital, Taipei, Taiwan (China)
- Department of Nuclear Medicine, Taipei Medical University and Hospital, Taipei, Taiwan (China)
Microtubules are part of cell structures that play a role in regulating the migration of cancer cells. The cellular apoptosis susceptibility (CSE1L/CAS) protein is a microtubule-associated protein that is highly expressed in cancer. We report here that CSE1L regulates the association of {alpha}-tubulin with {beta}-tubulin and promotes the migration of MCF-7 breast cancer cells. CSE1L was associated with {alpha}-tubulin and {beta}-tubulin in GST (glutathione S-transferase) pull-down and immunoprecipitation assays. CSE1L-GFP (green fluorescence protein) fusion protein experiments showed that the N-terminal of CSE1L interacted with microtubules. Increased CSE1L expression resulted in decreased tyrosine phosphorylation of {alpha}-tubulin and {beta}-tubulin, increased {alpha}-tubulin and {beta}-tubulin association, and enhanced assembly of microtubules. Cell protrusions or pseudopodia are temporary extensions of the plasma membrane and are implicated in cancer cell migration and invasion. Increased CSE1L expression increased the extension of MCF-7 cell protrusions. In vitro migration assay showed that enhanced CSE1L expression increased the migration of MCF-7 cells. Our results indicate that CSE1L plays a role in regulating the extension of cell protrusions and promotes the migration of cancer cells.
- OSTI ID:
- 22209912
- Journal Information:
- Experimental Cell Research, Vol. 316, Issue 17; Other Information: Copyright (c) 2010 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); ISSN 0014-4827
- Country of Publication:
- United States
- Language:
- English
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