Parp2 is required for the differentiation of post-meiotic germ cells: Identification of a spermatid-specific complex containing Parp1, Parp2, TP2 and HSPA2

Journal Article · Thu Oct 01 00:00:00 EDT 2009 · Experimental Cell Research

DOI:https://doi.org/10.1016/J.YEXCR.2009.07.003· OSTI ID:22209807

Quenet, Delphine ^[1]; Mark, Manuel ^[2]; Govin, Jerome ^[3]; Dorsselear, A. van ^[4]; Schreiber, Valerie ^[1]; Khochbin, Saadi ^[3]; Dantzer, Francoise ^[1]

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1. IREBS-FRE 3211, Ecole Superieure de Biotechnologie de Strasbourg, F-67412 Illkirch cedex (France)
2. Institut de Genetique et de Biologie Moleculaire et Cellulaire (IGBMC), Institut Clinique de la souris (ICS), F-67404 Illkirch cedex (France)
3. INSERM, U823, Grenoble, F-38706 (France)
4. Laboratoire de Spectrometrie de Masse Bio-organique, UMR7178, Ecole de Chimie, Polymeres et Materiaux, Strasbourg (France)

Spermiogenesis is a complex male germ cell post-meiotic differentiation process characterized by dramatic changes in chromatin structure and function, including chromatin condensation, transcriptional inhibition and the sequential replacement of histones by transition proteins and protamines. Recent advances, in mammalian cells, suggest a possible role of poly(ADP-ribosyl)ation catalyzed by Parp1 and/or Parp2 in this process. We have recently reported severely compromised spermiogenesis in Parp2-deficient mice characterized by a marked delay in nuclear elongation whose molecular mechanisms remain however unknown. Here, using in vitro protein-protein interaction assays, we show that Parp2 interacts significantly with both the transition protein TP2 and the transition chaperone HSPA2, whereas Parp1 binds weakly to HSPA2. Parp2-TP2 interaction is partly mediated by poly(ADP-ribosyl)ation. Only Parp1 poly(ADP-ribosyl)ates HSPA2. In addition, a detailed analysis of spermatid maturation in Parp2-deficient mice, combining immunohistochemistry and electron microscopic approaches, reveals a loss of spermatids expressing TP2, a defect in chromatin condensation and abnormal formation of the manchette microtubules that, together, contribute to spermatid-specific cell death. In conclusion, we propose both Parps as new participants of a spermatid-specific protein complex involved in genome reorganization throughout spermiogenesis.

• OSTI ID: 22209807
• Journal Information: Experimental Cell Research, Vol. 315, Issue 16; Other Information: Copyright (c) 2009 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); ISSN 0014-4827
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
ADP
APOPTOSIS
CHROMATIN
HISTONES
IN VITRO
MICE
MICROTUBULES
PROTAMINES
RIBOSE
SPERMATOZOA