KLF10, transforming growth factor-{beta}-inducible early gene 1, acts as a tumor suppressor

Journal Article · Fri Mar 09 00:00:00 EST 2012 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/J.BBRC.2012.02.032· OSTI ID:22207748

Song, Ki-Duk ^[1]; Kim, Duk-Jung ^[2]; Lee, Jong Eun ^[3]; Yun, Cheol-Heui ^[1]

---

1. Center for Agricultural Biomaterials, Seoul National University, Seoul 151-921 (Korea, Republic of)
2. The Institute of Hankook Life Science, 7-9 Myungryun-dong, Jongno-gu, Seoul 110-521 (Korea, Republic of)
3. Department of Anatomy, College of Medicine, Yonsei University, Seoul 120-752 (Korea, Republic of)

Highlights: Black-Right-Pointing-Pointer KLF10{sup -/-} mice exhibited accelerated papilloma development after DMBA/TPA treatment. Black-Right-Pointing-Pointer KLF10{sup -/-} keratinocytes showed increased proliferation and apoptosis. Black-Right-Pointing-Pointer KLF10{sup -/-} MEFs yielded more colonies than wild-type one with H-Ras transfection. Black-Right-Pointing-Pointer KLF10 dose-dependently activated p21{sup WAF1/CIP1} transcription. Black-Right-Pointing-Pointer KLF10 is a tumor suppressor and that it targets p21{sup WAF1/CIP1} transcription. -- Abstract: Krueppel-like factor 10 (KLF10) has been suggested to be a putative tumor suppressor. In the present study, we generated KLF10 deficient mice to explore this hypothesis in vivo. KLF10 deficient mice exhibited increased predisposition to skin tumorigenesis and markedly accelerated papilloma development after DMBA/TPA treatment. On the other hand, KLF10 deficient keratinocytes showed increased proliferation and apoptosis. In colony formation assays after oncogenic H-Ras transfection, KLF10 deficient mouse embryonic fibroblasts (MEFs) yielded more colonies than wild-type MEFs. Furthermore, KLF10 dose-dependently activated p21{sup WAF1/CIP1} transcription, which was independent of p53 and Sp1 binding sites in p21{sup WAF1/CIP1} promoter. This study demonstrates that KLF10 is a tumor suppressor and that it targets p21{sup WAF1/CIP1} transcription.

• OSTI ID: 22207748
• Journal Information: Biochemical and Biophysical Research Communications, Vol. 419, Issue 2; Other Information: Copyright (c) 2012 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
APOPTOSIS
COLONY FORMATION
DIMETHYLBENZANTHRACENE
DOSES
FIBROBLASTS
GENES
GROWTH FACTORS
IN VIVO
MICE
MONOCLINIC LATTICES
NEOPLASMS
SKIN
TRANSCRIPTION