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Title: The role of Zic family zinc finger transcription factors in the proliferation and differentiation of retinal progenitor cells

Abstract

Highlights: Black-Right-Pointing-Pointer Zic transcription factors expressed early retinal progenitor cells. Black-Right-Pointing-Pointer Zics sustain proliferation activity of retinal progenitor cells. Black-Right-Pointing-Pointer Overexpression of Zic in retinal progenitors perturbed rod differentiation. Black-Right-Pointing-Pointer Fate determination to rod photoreceptor was not affected. -- Abstract: Members of the Zic family of zinc finger transcription factors play critical roles in a variety of developmental processes. Using DNA microarray analysis, we found that Zics are strongly expressed in SSEA-1-positive early retinal progenitors in the peripheral region of the mouse retina. Reverse-transcription polymerase chain reaction using mRNA from the retina at various developmental stages showed that Zic1 and Zic2 are expressed in the embryonic retina and then gradually disappear during retinal development. Zic3 is also expressed in the embryonic retina; its expression level slightly decreases but it is expressed until adulthood. We overexpressed Zic1, Zic2, or Zic3 in retinal progenitors at embryonic day 17.5 and cultured the retina as explants for 2 weeks. The number of rod photoreceptors was fewer than in the control, but no other cell types showed significant differences between control and Zic overexpressing cells. The proliferation activity of normal retinal progenitors decreased after 5 days in culture, as observed in normal in vivo developmentalmore » processes. However, Zic expressing retinal cells continued to proliferate at days 5 and 7, suggesting that Zics sustain the proliferation activities of retinal progenitor cells. Since the effects of Zic1, 2, and 3 are indistinguishable in terms of differentiation and proliferation of retinal progenitors, the redundant function of Zics in retinal development is suggested.« less

Authors:
 [1];  [1];  [2];  [3];  [1]
  1. Division of Molecular and Developmental Biology, Institute of Medical Science, University of Tokyo (Japan)
  2. Division of Stem Cell Therapy, Center for Stem Cell Biology and Regenerative Medicine, Institute of Medical Science, University of Tokyo (Japan)
  3. Department of Ophthalmology, Teikyo University School of Medicine, Tokyo (Japan)
Publication Date:
OSTI Identifier:
22207563
Resource Type:
Journal Article
Journal Name:
Biochemical and Biophysical Research Communications
Additional Journal Information:
Journal Volume: 415; Journal Issue: 1; Other Information: Copyright (c) 2011 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; CELL PROLIFERATION; DNA; IN VIVO; MESSENGER-RNA; MICE; POLYMERASE CHAIN REACTION; RETINA; TRANSCRIPTION; TRANSCRIPTION FACTORS

Citation Formats

Watabe, Yui, Department of Ophthalmology, Teikyo University School of Medicine, Tokyo, Division of Orthoptics, Teikyo University School of Medical Care and Technology, Tokyo, Baba, Yukihiro, Nakauchi, Hiromitsu, Mizota, Atsushi, and Watanabe, Sumiko. The role of Zic family zinc finger transcription factors in the proliferation and differentiation of retinal progenitor cells. United States: N. p., 2011. Web. doi:10.1016/J.BBRC.2011.10.007.
Watabe, Yui, Department of Ophthalmology, Teikyo University School of Medicine, Tokyo, Division of Orthoptics, Teikyo University School of Medical Care and Technology, Tokyo, Baba, Yukihiro, Nakauchi, Hiromitsu, Mizota, Atsushi, & Watanabe, Sumiko. The role of Zic family zinc finger transcription factors in the proliferation and differentiation of retinal progenitor cells. United States. https://doi.org/10.1016/J.BBRC.2011.10.007
Watabe, Yui, Department of Ophthalmology, Teikyo University School of Medicine, Tokyo, Division of Orthoptics, Teikyo University School of Medical Care and Technology, Tokyo, Baba, Yukihiro, Nakauchi, Hiromitsu, Mizota, Atsushi, and Watanabe, Sumiko. 2011. "The role of Zic family zinc finger transcription factors in the proliferation and differentiation of retinal progenitor cells". United States. https://doi.org/10.1016/J.BBRC.2011.10.007.
@article{osti_22207563,
title = {The role of Zic family zinc finger transcription factors in the proliferation and differentiation of retinal progenitor cells},
author = {Watabe, Yui and Department of Ophthalmology, Teikyo University School of Medicine, Tokyo and Division of Orthoptics, Teikyo University School of Medical Care and Technology, Tokyo and Baba, Yukihiro and Nakauchi, Hiromitsu and Mizota, Atsushi and Watanabe, Sumiko},
abstractNote = {Highlights: Black-Right-Pointing-Pointer Zic transcription factors expressed early retinal progenitor cells. Black-Right-Pointing-Pointer Zics sustain proliferation activity of retinal progenitor cells. Black-Right-Pointing-Pointer Overexpression of Zic in retinal progenitors perturbed rod differentiation. Black-Right-Pointing-Pointer Fate determination to rod photoreceptor was not affected. -- Abstract: Members of the Zic family of zinc finger transcription factors play critical roles in a variety of developmental processes. Using DNA microarray analysis, we found that Zics are strongly expressed in SSEA-1-positive early retinal progenitors in the peripheral region of the mouse retina. Reverse-transcription polymerase chain reaction using mRNA from the retina at various developmental stages showed that Zic1 and Zic2 are expressed in the embryonic retina and then gradually disappear during retinal development. Zic3 is also expressed in the embryonic retina; its expression level slightly decreases but it is expressed until adulthood. We overexpressed Zic1, Zic2, or Zic3 in retinal progenitors at embryonic day 17.5 and cultured the retina as explants for 2 weeks. The number of rod photoreceptors was fewer than in the control, but no other cell types showed significant differences between control and Zic overexpressing cells. The proliferation activity of normal retinal progenitors decreased after 5 days in culture, as observed in normal in vivo developmental processes. However, Zic expressing retinal cells continued to proliferate at days 5 and 7, suggesting that Zics sustain the proliferation activities of retinal progenitor cells. Since the effects of Zic1, 2, and 3 are indistinguishable in terms of differentiation and proliferation of retinal progenitors, the redundant function of Zics in retinal development is suggested.},
doi = {10.1016/J.BBRC.2011.10.007},
url = {https://www.osti.gov/biblio/22207563}, journal = {Biochemical and Biophysical Research Communications},
issn = {0006-291X},
number = 1,
volume = 415,
place = {United States},
year = {Fri Nov 11 00:00:00 EST 2011},
month = {Fri Nov 11 00:00:00 EST 2011}
}