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Title: Roles of ATR1 paralogs YMR279c and YOR378w in boron stress tolerance

Abstract

Highlights: {yields} ATR1 paralog YMR279c plays role in boron detoxification. {yields} YMR279c overexpression lowers cytoplasmic boron levels. {yields} ATR1 paralog YOR378w has no roles in boron stress response. -- Abstract: Boron is a necessary nutrient for plants and animals, however excess of it causes toxicity. Previously, Atr1 and Arabidopsis Bor1 homolog were identified as the boron efflux pump in yeast, which lower the cytosolic boron concentration and help cells to survive in the presence of toxic amount of boron. In this study, we analyzed ATR1 paralogs, YMR279c and YOR378w, to understand whether they participate in boron stress tolerance in yeast. Even though these genes share homology with ATR1, neither their deletion rendered cells boron sensitive nor their expression was significantly upregulated by boron treatment. However, expression of YMR279, but not YOR378w, from the constitutive GAPDH promoter on a high copy plasmid provided remarkable boron resistance by decreasing intracellular boron levels. Thus our results suggest the presence of a third boron exporter, YMR279c, which functions similar to ATR1 and provides boron resistance in yeast.

Authors:
; ; ;  [1];  [1]
  1. Izmir Institute of Technology, Department of Molecular Biology and Genetics, 35430 Urla, Izmir (Turkey)
Publication Date:
OSTI Identifier:
22204963
Resource Type:
Journal Article
Journal Name:
Biochemical and Biophysical Research Communications
Additional Journal Information:
Journal Volume: 409; Journal Issue: 4; Other Information: Copyright (c) 2011 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ARABIDOPSIS; BIOLOGICAL STRESS; BORIC ACID; BORON; CONCENTRATION RATIO; DETOXIFICATION; GENES; NUTRIENTS; TOLERANCE; TOXICITY; YEASTS

Citation Formats

Bozdag, Gonensin Ozan, Uluisik, Irem, Gulculer, Gulce Sila, Karakaya, Huseyin C., and Koc, Ahmet. Roles of ATR1 paralogs YMR279c and YOR378w in boron stress tolerance. United States: N. p., 2011. Web. doi:10.1016/J.BBRC.2011.05.080.
Bozdag, Gonensin Ozan, Uluisik, Irem, Gulculer, Gulce Sila, Karakaya, Huseyin C., & Koc, Ahmet. Roles of ATR1 paralogs YMR279c and YOR378w in boron stress tolerance. United States. https://doi.org/10.1016/J.BBRC.2011.05.080
Bozdag, Gonensin Ozan, Uluisik, Irem, Gulculer, Gulce Sila, Karakaya, Huseyin C., and Koc, Ahmet. 2011. "Roles of ATR1 paralogs YMR279c and YOR378w in boron stress tolerance". United States. https://doi.org/10.1016/J.BBRC.2011.05.080.
@article{osti_22204963,
title = {Roles of ATR1 paralogs YMR279c and YOR378w in boron stress tolerance},
author = {Bozdag, Gonensin Ozan and Uluisik, Irem and Gulculer, Gulce Sila and Karakaya, Huseyin C. and Koc, Ahmet},
abstractNote = {Highlights: {yields} ATR1 paralog YMR279c plays role in boron detoxification. {yields} YMR279c overexpression lowers cytoplasmic boron levels. {yields} ATR1 paralog YOR378w has no roles in boron stress response. -- Abstract: Boron is a necessary nutrient for plants and animals, however excess of it causes toxicity. Previously, Atr1 and Arabidopsis Bor1 homolog were identified as the boron efflux pump in yeast, which lower the cytosolic boron concentration and help cells to survive in the presence of toxic amount of boron. In this study, we analyzed ATR1 paralogs, YMR279c and YOR378w, to understand whether they participate in boron stress tolerance in yeast. Even though these genes share homology with ATR1, neither their deletion rendered cells boron sensitive nor their expression was significantly upregulated by boron treatment. However, expression of YMR279, but not YOR378w, from the constitutive GAPDH promoter on a high copy plasmid provided remarkable boron resistance by decreasing intracellular boron levels. Thus our results suggest the presence of a third boron exporter, YMR279c, which functions similar to ATR1 and provides boron resistance in yeast.},
doi = {10.1016/J.BBRC.2011.05.080},
url = {https://www.osti.gov/biblio/22204963}, journal = {Biochemical and Biophysical Research Communications},
issn = {0006-291X},
number = 4,
volume = 409,
place = {United States},
year = {Fri Jun 17 00:00:00 EDT 2011},
month = {Fri Jun 17 00:00:00 EDT 2011}
}