Membrane fusion inducers, chloroquine and spermidine increase lipoplex-mediated gene transfection
- Biochemistry Department, National Polytechnic Institute (IPN), Mexico City 11340 (Mexico)
- Department of Genetics and Molecular Biology, Centre for Research and Advanced Studies (CINVESTAV), IPN, Mexico City 07360 (Mexico)
Gene transfection into mammalian cells can be achieved with viral and non-viral vectors. Non-viral vectors, such as cationic lipids that form lipoplexes with DNA, are safer and more stable than viral vectors, but their transfection efficiencies are lower. Here we describe that the simultaneous treatment with a membrane fusion inducer (chlorpromazine or procainamide) plus the lysosomotropic agent chloroquine increases lipoplex-mediated gene transfection in human (HEK293 and C-33 A) and rat (PC12) cell lines (up to 9.2-fold), as well as in situ in BALB/c mice spleens and livers (up to 6-fold); and that the polyamine spermidine increases lipoplex-mediated gene transfection and expression in cell cultures. The use of these four drugs provides a novel, safe and relatively inexpensive way to considerably increase lipoplex-mediated gene transfection efficiency.
- OSTI ID:
- 22202627
- Journal Information:
- Biochemical and Biophysical Research Communications, Vol. 396, Issue 2; Other Information: Copyright (c) 2010 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
- Country of Publication:
- United States
- Language:
- English
Similar Records
Cationic Phospholipids Forming Cubic Phases: Lipoplex Structure and Transfection Efficiency
Functionalization with C-terminal cysteine enhances transfection efficiency of cell-penetrating peptides through dimer formation