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Title: Bis(monoacylglycero)phosphate and ganglioside GM1 spontaneously form small homogeneous vesicles at specific concentrations

Abstract

The morphology and size of hydrated lipid dispersions of bis(monoacylglycero)phosphate (BMP) mixed with varying mole percentages of the ganglioside GM1 were investigated by dynamic light scattering (DLS) and transmission electron microscopy (TEM). Electron paramagnetic resonance (EPR) spectroscopy of these same mixtures, doped at 0.5 mol% with doxyl labeled lipids, was used to investigate acyl-chain packing. Results show that for 20-30% GM1, hydrated BMP:GM1 mixtures spontaneously form small spherical vesicles with diameters {approx}100 nm and a narrow size distribution profile. For other concentrations of GM1, hydrated dispersions with BMP have non-spherical shapes and heterogeneous size profiles, with average vesicle diameters >400 nm. All samples were prepared at pH 5.5 to mimic the lumen acidity of the late endosome where BMP is an essential component of intraendosomal vesicle budding, lipid sorting and trafficking. These findings indicate that GM1 and BMP under a limited concentration range spontaneously form small vesicles of homogeneous size in an energy independent manner without the need of protein templating. Because BMP is essential for intraendosomal vesicle formation, these results imply that lipid-lipid interactions may play a critical role in the endosomal process of lipid sorting and trafficking.

Authors:
; ;  [1]
  1. Department of Chemistry, P.O. Box 117200, University of Florida, Gainesville, FL 32611-7200 (United States)
Publication Date:
OSTI Identifier:
22202451
Resource Type:
Journal Article
Journal Name:
Biochemical and Biophysical Research Communications
Additional Journal Information:
Journal Volume: 394; Journal Issue: 3; Other Information: Copyright (c) 2010 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; BIOPHYSICS; CONCENTRATION RATIO; DOPED MATERIALS; ELECTRON SPIN RESONANCE; LIGHT SCATTERING; LIPIDS; MORPHOLOGY; PH VALUE; PHOSPHATES; PROTEINS; SORTING; SPECTROSCOPY; TRANSMISSION ELECTRON MICROSCOPY

Citation Formats

Chebukati, Janetricks N., Goff, Philip C., Frederick, Thomas E., and Fanucci, Gail E., E-mail: fanucci@chem.ufl.edu. Bis(monoacylglycero)phosphate and ganglioside GM1 spontaneously form small homogeneous vesicles at specific concentrations. United States: N. p., 2010. Web. doi:10.1016/J.BBRC.2010.02.180.
Chebukati, Janetricks N., Goff, Philip C., Frederick, Thomas E., & Fanucci, Gail E., E-mail: fanucci@chem.ufl.edu. Bis(monoacylglycero)phosphate and ganglioside GM1 spontaneously form small homogeneous vesicles at specific concentrations. United States. https://doi.org/10.1016/J.BBRC.2010.02.180
Chebukati, Janetricks N., Goff, Philip C., Frederick, Thomas E., and Fanucci, Gail E., E-mail: fanucci@chem.ufl.edu. 2010. "Bis(monoacylglycero)phosphate and ganglioside GM1 spontaneously form small homogeneous vesicles at specific concentrations". United States. https://doi.org/10.1016/J.BBRC.2010.02.180.
@article{osti_22202451,
title = {Bis(monoacylglycero)phosphate and ganglioside GM1 spontaneously form small homogeneous vesicles at specific concentrations},
author = {Chebukati, Janetricks N. and Goff, Philip C. and Frederick, Thomas E. and Fanucci, Gail E., E-mail: fanucci@chem.ufl.edu},
abstractNote = {The morphology and size of hydrated lipid dispersions of bis(monoacylglycero)phosphate (BMP) mixed with varying mole percentages of the ganglioside GM1 were investigated by dynamic light scattering (DLS) and transmission electron microscopy (TEM). Electron paramagnetic resonance (EPR) spectroscopy of these same mixtures, doped at 0.5 mol% with doxyl labeled lipids, was used to investigate acyl-chain packing. Results show that for 20-30% GM1, hydrated BMP:GM1 mixtures spontaneously form small spherical vesicles with diameters {approx}100 nm and a narrow size distribution profile. For other concentrations of GM1, hydrated dispersions with BMP have non-spherical shapes and heterogeneous size profiles, with average vesicle diameters >400 nm. All samples were prepared at pH 5.5 to mimic the lumen acidity of the late endosome where BMP is an essential component of intraendosomal vesicle budding, lipid sorting and trafficking. These findings indicate that GM1 and BMP under a limited concentration range spontaneously form small vesicles of homogeneous size in an energy independent manner without the need of protein templating. Because BMP is essential for intraendosomal vesicle formation, these results imply that lipid-lipid interactions may play a critical role in the endosomal process of lipid sorting and trafficking.},
doi = {10.1016/J.BBRC.2010.02.180},
url = {https://www.osti.gov/biblio/22202451}, journal = {Biochemical and Biophysical Research Communications},
issn = {0006-291X},
number = 3,
volume = 394,
place = {United States},
year = {Fri Apr 09 00:00:00 EDT 2010},
month = {Fri Apr 09 00:00:00 EDT 2010}
}