Arabidopsis dynamin-related protein 1A polymers bind, but do not tubulate, liposomes
Abstract
The Arabidopsis dynamin-related protein 1A (AtDRP1A) is involved in endocytosis and cell plate maturation in Arabidopsis. Unlike dynamin, AtDRP1A does not have any recognized membrane binding or protein-protein interaction domains. We report that GTPase active AtDRP1A purified from Escherichia coli as a fusion to maltose binding protein forms homopolymers visible by negative staining electron microscopy. These polymers interact with protein-free liposomes whose lipid composition mimics that of the inner leaflet of the Arabidopsis plasma membrane, suggesting that lipid-binding may play a role in AtDRP1A function. However, AtDRP1A polymers do not appear to assemble and disassemble in a dynamic fashion and do not have the ability to tubulate liposomes in vitro, suggesting that additional factors or modifications are necessary for AtDRP1A's in vivo function.
- Authors:
-
- Department of Biochemistry, University of Wisconsin - Madison, 433 Babcock Dr., Madison, WI 53706 (United States)
- Publication Date:
- OSTI Identifier:
- 22202422
- Resource Type:
- Journal Article
- Journal Name:
- Biochemical and Biophysical Research Communications
- Additional Journal Information:
- Journal Volume: 393; Journal Issue: 4; Other Information: Copyright (c) 2010 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 60 APPLIED LIFE SCIENCES; ARABIDOPSIS; CHOLINE; ELECTRON MICROSCOPY; ESCHERICHIA COLI; GLYCEROL; IN VITRO; IN VIVO; INOSITOL; LIPIDS; LIPOSOMES; MALTOSE; PH VALUE; PHOSPHATES; POLYMERS; PROLINE; PROTEINS; SERINE; SOYBEANS; TOBACCO; VIRUSES
Citation Formats
Backues, Steven K., and Bednarek, Sebastian Y., E-mail: sybednar@wisc.edu. Arabidopsis dynamin-related protein 1A polymers bind, but do not tubulate, liposomes. United States: N. p., 2010.
Web. doi:10.1016/J.BBRC.2010.02.070.
Backues, Steven K., & Bednarek, Sebastian Y., E-mail: sybednar@wisc.edu. Arabidopsis dynamin-related protein 1A polymers bind, but do not tubulate, liposomes. United States. https://doi.org/10.1016/J.BBRC.2010.02.070
Backues, Steven K., and Bednarek, Sebastian Y., E-mail: sybednar@wisc.edu. 2010.
"Arabidopsis dynamin-related protein 1A polymers bind, but do not tubulate, liposomes". United States. https://doi.org/10.1016/J.BBRC.2010.02.070.
@article{osti_22202422,
title = {Arabidopsis dynamin-related protein 1A polymers bind, but do not tubulate, liposomes},
author = {Backues, Steven K. and Bednarek, Sebastian Y., E-mail: sybednar@wisc.edu},
abstractNote = {The Arabidopsis dynamin-related protein 1A (AtDRP1A) is involved in endocytosis and cell plate maturation in Arabidopsis. Unlike dynamin, AtDRP1A does not have any recognized membrane binding or protein-protein interaction domains. We report that GTPase active AtDRP1A purified from Escherichia coli as a fusion to maltose binding protein forms homopolymers visible by negative staining electron microscopy. These polymers interact with protein-free liposomes whose lipid composition mimics that of the inner leaflet of the Arabidopsis plasma membrane, suggesting that lipid-binding may play a role in AtDRP1A function. However, AtDRP1A polymers do not appear to assemble and disassemble in a dynamic fashion and do not have the ability to tubulate liposomes in vitro, suggesting that additional factors or modifications are necessary for AtDRP1A's in vivo function.},
doi = {10.1016/J.BBRC.2010.02.070},
url = {https://www.osti.gov/biblio/22202422},
journal = {Biochemical and Biophysical Research Communications},
issn = {0006-291X},
number = 4,
volume = 393,
place = {United States},
year = {Fri Mar 19 00:00:00 EDT 2010},
month = {Fri Mar 19 00:00:00 EDT 2010}
}