The influence of Pyk2 on the mechanical properties in fibroblasts

Journal Article · Fri Mar 19 00:00:00 EDT 2010 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/J.BBRC.2010.02.059· OSTI ID:22202420

Klemm, Anna H.; Kienle, Sandra ^[1]; Rheinlaender, Johannes; Schaeffer, Tilman E. ^[2]

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1. Center for Medical Physics and Technology, Biophysics Group, Friedrich-Alexander-University, Erlangen-Nuremberg, Erlangen (Germany)
2. Insitute of Applied Physics, Friedrich-Alexander-University, Erlangen-Nuremberg, Erlangen (Germany)

The cell surface receptor integrin is involved in signaling mechanical stresses via the focal adhesion complex (FAC) into the cell. Within FAC, the focal adhesion kinase (FAK) and Pyk2 are believed to act as important scaffolding proteins. Based on the knowledge that many signal transducing molecules are transiently immobilized within FAC connecting the cytoskeleton with integrins, we applied magnetic tweezer and atomic force microscopic measurements to determine the influence of FAK and Pyk2 in cells mechanically. Using mouse embryonic fibroblasts (MEF; FAK{sup +/+}, FAK{sup -/-}, and siRNA-Pyk2 treated FAK{sup -/-} cells) provided a unique opportunity to describe the function of FAK and Pyk2 in more detail and to define their influence on FAC and actin distribution.

• OSTI ID: 22202420
• Journal Information: Biochemical and Biophysical Research Communications, Vol. 393, Issue 4; Other Information: Copyright (c) 2010 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
ACTIN
ATOMIC FORCE MICROSCOPY
FIBROBLASTS
MECHANICAL PROPERTIES
MICE
MICROTUBULES
MOLECULES
RECEPTORS