Spred2 is involved in imatinib-induced cytotoxicity in chronic myeloid leukemia cells

Journal Article · Fri Mar 19 00:00:00 EDT 2010 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/J.BBRC.2010.02.044· OSTI ID:22202416

Liu, Xiao-Yun; Yang, Yue-Feng; Wu, Chu-Tse; Xiao, Feng-Jun; Zhang, Qun-Wei ^[1]; Ma, Xiao-Ni ^[2]; Li, Qing-Fang; Yan, Jun ^[1]; Wang, Hua ^[1]; Wang, Li-Sheng ^[1]

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1. Department of Experimental Hematology, Beijing Institute of Radiation Medicine, Beijing 100850 (China)
2. Lanzhou University of Technology, Lanzhou 730050 (China)

Spreds, a recently established class of negative regulators of the Ras-ERK (extracellular signal-regulated kinase) pathway, are involved in hematogenesises, allergic disorders and tumourigenesis. However, their role in hematologic neoplasms is largely unknown. Possible effects of Spreds on other signal pathways closely related to Ras-ERK have been poorly investigated. In this study, we investigated the in vitro effects of Spred2 on chronic myeloid leukemia (CML) cells. In addition to inhibiting the well-established Ras-ERK cascade, adenovirus-mediated Spred2 over-expression inhibits constitutive and stem cell factor (SCF)-stimulated sphingosine kinase-1 (SPHK1) and Mcl-1 expression, as well as inhibiting proliferation and inducing apoptosis in CML cells. In K562 cells and primary CML cells, imatinib induces endogenous Spred2 expression. Spred2 silencing by stable RNA interference partly protects K562 cells against imatinib-induced apoptosis. Together, these data implicate Spred2 in imatinib-induced cytotoxicity in CML cells, possibly by inhibiting the Ras-ERK cascade and the pro-survival signaling molecules SPHK1 and Mcl-1. These findings reveal potential targets for selective therapy of CML.

• OSTI ID: 22202416
• Journal Information: Biochemical and Biophysical Research Communications, Vol. 393, Issue 4; Other Information: Copyright (c) 2010 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
ADENOVIRUS
APOPTOSIS
CELL PROLIFERATION
MOLECULES
MYELOID LEUKEMIA
RNA
SIGNALS
STEM CELLS
THERAPY
TOXICITY