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Title: Cleavage-induced termination in U2 snRNA gene expression

Abstract

The maturation of many small nuclear RNAs is dependent on RNase III-like endonuclease mediated cleavage, which generates a loading site for the exosome complex that trims the precursor at its 3' end. Using a temperature sensitive Pac1 nuclease, here we show that the endonuclease cleavage is equally important in terminating the transcription of the U2 snRNA in Schizosaccharomyces pombe. Using a temperature sensitive Dhp1p 5' {yields} 3' exonuclease, we demonstrate that it also is an essential component of the termination pathway. Taken together the results support a 'reversed torpedoes' model for the termination and maturation of the U2 snRNA; the Pac1 endonuclease cleavage provides entry sites for the 3' and 5' exonuclease activities, leading to RNA maturation in one direction and transcript termination in the other.

Authors:
 [1]
  1. Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario, Canada N1G 2W1 (Canada)
Publication Date:
OSTI Identifier:
22202409
Resource Type:
Journal Article
Journal Name:
Biochemical and Biophysical Research Communications
Additional Journal Information:
Journal Volume: 393; Journal Issue: 3; Other Information: Copyright (c) 2010 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; CLEAVAGE; GENES; LOADING; PRECURSOR; RNA; TRANSCRIPTION

Citation Formats

Nabavi, Sadeq, and Nazar, Ross N., E-mail: rnnazar@uoguelph.ca. Cleavage-induced termination in U2 snRNA gene expression. United States: N. p., 2010. Web. doi:10.1016/J.BBRC.2010.02.023.
Nabavi, Sadeq, & Nazar, Ross N., E-mail: rnnazar@uoguelph.ca. Cleavage-induced termination in U2 snRNA gene expression. United States. https://doi.org/10.1016/J.BBRC.2010.02.023
Nabavi, Sadeq, and Nazar, Ross N., E-mail: rnnazar@uoguelph.ca. 2010. "Cleavage-induced termination in U2 snRNA gene expression". United States. https://doi.org/10.1016/J.BBRC.2010.02.023.
@article{osti_22202409,
title = {Cleavage-induced termination in U2 snRNA gene expression},
author = {Nabavi, Sadeq and Nazar, Ross N., E-mail: rnnazar@uoguelph.ca},
abstractNote = {The maturation of many small nuclear RNAs is dependent on RNase III-like endonuclease mediated cleavage, which generates a loading site for the exosome complex that trims the precursor at its 3' end. Using a temperature sensitive Pac1 nuclease, here we show that the endonuclease cleavage is equally important in terminating the transcription of the U2 snRNA in Schizosaccharomyces pombe. Using a temperature sensitive Dhp1p 5' {yields} 3' exonuclease, we demonstrate that it also is an essential component of the termination pathway. Taken together the results support a 'reversed torpedoes' model for the termination and maturation of the U2 snRNA; the Pac1 endonuclease cleavage provides entry sites for the 3' and 5' exonuclease activities, leading to RNA maturation in one direction and transcript termination in the other.},
doi = {10.1016/J.BBRC.2010.02.023},
url = {https://www.osti.gov/biblio/22202409}, journal = {Biochemical and Biophysical Research Communications},
issn = {0006-291X},
number = 3,
volume = 393,
place = {United States},
year = {Fri Mar 12 00:00:00 EST 2010},
month = {Fri Mar 12 00:00:00 EST 2010}
}