Geranylfarnesyl diphosphate synthase from Methanosarcina mazei: Different role, different evolution

Journal Article · Fri Feb 26 00:00:00 EST 2010 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/J.BBRC.2010.01.063· OSTI ID:22202388

Ogawa, Takuya; Yoshimura, Tohru ^[1]; Hemmi, Hisashi ^[1]

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1. Department of Applied Molecular Bioscience, Graduate School of Bioagricultural Sciences, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi 464-8601 (Japan)

The gene of (all-E) geranylfarnesyl diphosphate synthase that is responsible for the biosynthesis of methanophenazine, an electron carrier utilized for methanogenesis, was cloned from a methanogenic archaeon Methanosarcina mazei Goe1. The properties of the recombinant enzyme and the results of phylogenetic analysis suggest that the enzyme is closely related to (all-E) prenyl diphosphate synthases that are responsible for the biosynthesis of respiratory quinones, rather than to the enzymes involved in the biosynthesis of archaeal membrane lipids, including (all-E) geranylfarnesyl diphosphate synthase from a thermophilic archaeon.

• OSTI ID: 22202388
• Journal Information: Biochemical and Biophysical Research Communications, Vol. 393, Issue 1; Other Information: Copyright (c) 2010 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
BIOSYNTHESIS
CARRIERS
COENZYMES
ENZYMES
FARMS
GENES
LIPIDS
PHOSPHATES
QUINONES
THIN-LAYER CHROMATOGRAPHY