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Title: PSF3 marks malignant colon cancer and has a role in cancer cell proliferation

Abstract

PSF3 (partner of Sld five 3) is a member of the tetrameric complex termed GINS, composed of SLD5, PSF1, PSF2, and PSF3, and well-conserved evolutionarily. Previous studies suggested that some GINS complex members are upregulated in cancer, but PSF3 expression in colon carcinoma has not been investigated. Here, we established a mouse anti-PSF3 antibody, and examined PSF3 expression in human colon carcinoma cell lines and colon carcinoma specimens. We found that PSF3 is expressed in the crypt region in normal colonic mucosa and that many PSF3-positive cells co-expressed Ki-67. This suggests that PSF3-positivity of normal mucosa is associated with cell proliferation. Expression of the PSF3 protein was greater in carcinoma compared with the adjacent normal mucosa, and even stronger in high-grade malignancies, suggesting that it may be associated with colon cancer progression. PSF3 gene knock-down in human colon carcinoma cell lines resulted in growth inhibition characterized by delayed S-phase progression. These results suggest that PSF3 is a potential biomarker for diagnosis of progression in colon cancer and could be a new target for cancer therapy.

Authors:
 [1];  [1]; ;  [2];  [1]
  1. Department of Signal Transduction, Research Institute for Microbial Diseases, Osaka University, 3-1 Yamadaoka, Suita, Osaka 565-0871 (Japan)
  2. Department of Gastroenterological Surgery, Graduate School of Medicine, Osaka University, 2-2 Yamadaoka, Suita, 565-0871 (Japan)
Publication Date:
OSTI Identifier:
22202360
Resource Type:
Journal Article
Journal Name:
Biochemical and Biophysical Research Communications
Additional Journal Information:
Journal Volume: 392; Journal Issue: 2; Other Information: Copyright (c) 2010 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ANTIBODIES; BIOLOGICAL MARKERS; CARCINOMAS; CELL PROLIFERATION; COMPARATIVE EVALUATIONS; DIAGNOSIS; GENES; GROWTH; HUMAN POPULATIONS; LARGE INTESTINE; MICE; MUCOUS MEMBRANES; PROTEINS; THERAPY

Citation Formats

Nagahama, Yumi, Ueno, Masaya, Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, CA 90095, Haraguchi, Naotsugu, Mori, Masaki, and Takakura, Nobuyuki. PSF3 marks malignant colon cancer and has a role in cancer cell proliferation. United States: N. p., 2010. Web. doi:10.1016/J.BBRC.2009.12.174.
Nagahama, Yumi, Ueno, Masaya, Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, CA 90095, Haraguchi, Naotsugu, Mori, Masaki, & Takakura, Nobuyuki. PSF3 marks malignant colon cancer and has a role in cancer cell proliferation. United States. https://doi.org/10.1016/J.BBRC.2009.12.174
Nagahama, Yumi, Ueno, Masaya, Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, CA 90095, Haraguchi, Naotsugu, Mori, Masaki, and Takakura, Nobuyuki. 2010. "PSF3 marks malignant colon cancer and has a role in cancer cell proliferation". United States. https://doi.org/10.1016/J.BBRC.2009.12.174.
@article{osti_22202360,
title = {PSF3 marks malignant colon cancer and has a role in cancer cell proliferation},
author = {Nagahama, Yumi and Ueno, Masaya and Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, CA 90095 and Haraguchi, Naotsugu and Mori, Masaki and Takakura, Nobuyuki},
abstractNote = {PSF3 (partner of Sld five 3) is a member of the tetrameric complex termed GINS, composed of SLD5, PSF1, PSF2, and PSF3, and well-conserved evolutionarily. Previous studies suggested that some GINS complex members are upregulated in cancer, but PSF3 expression in colon carcinoma has not been investigated. Here, we established a mouse anti-PSF3 antibody, and examined PSF3 expression in human colon carcinoma cell lines and colon carcinoma specimens. We found that PSF3 is expressed in the crypt region in normal colonic mucosa and that many PSF3-positive cells co-expressed Ki-67. This suggests that PSF3-positivity of normal mucosa is associated with cell proliferation. Expression of the PSF3 protein was greater in carcinoma compared with the adjacent normal mucosa, and even stronger in high-grade malignancies, suggesting that it may be associated with colon cancer progression. PSF3 gene knock-down in human colon carcinoma cell lines resulted in growth inhibition characterized by delayed S-phase progression. These results suggest that PSF3 is a potential biomarker for diagnosis of progression in colon cancer and could be a new target for cancer therapy.},
doi = {10.1016/J.BBRC.2009.12.174},
url = {https://www.osti.gov/biblio/22202360}, journal = {Biochemical and Biophysical Research Communications},
issn = {0006-291X},
number = 2,
volume = 392,
place = {United States},
year = {Fri Feb 05 00:00:00 EST 2010},
month = {Fri Feb 05 00:00:00 EST 2010}
}