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Title: SUMOylated IRF-1 shows oncogenic potential by mimicking IRF-2

Interferon regulatory factor-1 (IRF-1) is an interferon-induced transcriptional activator that suppresses tumors by impeding cell proliferation. Recently, we demonstrated that the level of SUMOylated IRF-1 is elevated in tumor cells, and that SUMOylation of IRF-1 attenuates its tumor-suppressive function. Here we report that SUMOylated IRF-1 mimics IRF-2, an antagonistic repressor, and shows oncogenic potential. To demonstrate the role of SUMOylated IRF-1 in tumorigenesis, we used SUMO-IRF-1 recombinant protein. Stable expression of SUMO-IRF-1 in NIH3T3 cells resulted in focus formation and anchorage-independent growth in soft agar. Inoculation of SUMO-IRF-1-transfected cells into athymic nude mice resulted in tumor formation and infiltration of adipose tissues. Finally, we demonstrated that SUMO-IRF-1 transforms NIH3T3 cells in a dose-dependent manner suggesting that SUMOylated IRF-1 may act as an oncogenic protein in tumor cells.
Authors:
 [1] ;  [2] ;  [3] ;  [1] ;  [4] ; ;  [5] ;  [6] ;  [7] ;  [1]
  1. Yonsei University, Division of Biological Science and Technology, Wonju 220-100 (Korea, Republic of)
  2. (Korea, Republic of)
  3. Clinical Trial Center for Functional Foods, Chonbuk National University Hospital, Jeonju 561-712 (Korea, Republic of)
  4. Department of Life Science, Dongguk Univ-Seoul, Seoul 100-715 (Korea, Republic of)
  5. Proteome Research Team, Korea Basic Science Institute, Daejeon 305-333 (Korea, Republic of)
  6. School of Biological Sciences and Biotechnology, Chonnam National University, Gwangju 500-757 (Korea, Republic of)
  7. Molecular Therapy Research Center, Sungkyunkwan University, Seoul 135-710 (Korea, Republic of)
Publication Date:
OSTI Identifier:
22199994
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 391; Journal Issue: 1; Other Information: Copyright (c) 2009 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ADIPOSE TISSUE; AGAR; CELL PROLIFERATION; DOSES; INOCULATION; INTERFERON; MICE; NEOPLASMS; TUMOR CELLS