Casein kinase II is required for the spindle assembly checkpoint by regulating Mad2p in fission yeast

Journal Article · Fri Oct 23 00:00:00 EDT 2009 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/J.BBRC.2009.08.030· OSTI ID:22199847

Shimada, Midori ^[1]; Yamamoto, Ayumu ^[2]; Murakami-Tonami, Yuko; Nakanishi, Makoto; Yoshida, Takashi ^[1]; Aiba, Hirofumi ^[3]; Murakami, Hiroshi ^[1]

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1. Department of Biochemistry and Cell Biology, Graduate School of Medicine, Nagoya City University, 1 Kawasumi, Mizuho-cho, Mizuho-ku, Nagoya 467-8601 (Japan)
2. Department of Chemistry, Shizuoka University, 836 Ohya, Suruga-ku, Sizuoka 422-8529 (Japan)
3. Laboratory of Molecular Microbiology, School of Agriculture, Nagoya University, Chikusa-ku, Nagoya 464-8601 (Japan)

The spindle checkpoint is a surveillance mechanism that ensures the fidelity of chromosome segregation in mitosis. Here we show that fission yeast casein kinase II (CK2) is required for this checkpoint function. In the CK2 mutants mitosis occurs in the presence of a spindle defect, and the spindle checkpoint protein Mad2p fails to localize to unattached kinetochores. The CK2 mutants are sensitive to the microtubule depolymerising drug thiabendazole, which is counteracted by ectopic expression of mad2{sup +}. The level of Mad2p is low in the CK2 mutants. These results suggest that CK2 has a role in the spindle checkpoint by regulating Mad2p.

• OSTI ID: 22199847
• Journal Information: Biochemical and Biophysical Research Communications, Vol. 388, Issue 3; Other Information: Copyright (c) 2009 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
CASEIN
CELL CYCLE
CHROMOSOMES
DRUGS
MEDICAL SURVEILLANCE
MICROTUBULES
MITOSIS
MUTANTS
YEASTS