Resveratrol enhances ultraviolet B-induced cell death through nuclear factor-{kappa}B pathway in human epidermoid carcinoma A431 cells
Abstract
Resveratrol has been reported to suppress cancer progression in several in vivo and in vitro models, whereas ultraviolet B (UVB), a major risk for skin cancer, is known to induce cell death in cancerous cells. Here, we investigated whether resveratrol can sensitize A431 human epidermoid carcinoma cells to UVB-induced cell death. We examined the combined effect of UVB (30 mJ/cm{sup 2}) and resveratrol (60 {mu}M) on A431 cells. Exposure of A431 carcinoma cells to UVB radiation or resveratrol can inhibit cell proliferation and induce apoptosis. However, the combination of resveratrol and UVB exposure was associated with increased proliferation inhibition of A431 cells compared with either agent alone. Furthermore, results showed that resveratrol and UVB treatment of A431 cells disrupted the nuclear factor-kappaB (NF-{kappa}B) pathway by blocking phosphorylation of serine 536 and inactivating NF-{kappa}B and subsequent degradation of I{kappa}B{alpha}, which regulates the expression of survivin. Resveratrol and UVB treatment also decreased the phosphorylation of tyrosine 701 of the important transcription factor signal transducer activator of transcription (STAT1), which in turn inhibited translocation of phospho-STAT1 to the nucleus. Moreover, resveratrol/UVB also inhibited the metastatic protein LIMK1, which reduced the motility of A431 cells. In conclusion, our study demonstrates that the combination ofmore »
- Authors:
-
- Proteomics Laboratory, Indian Institute of Toxicology Research (CSIR), P.O. Box 80, M.G. Marg, Lucknow 226 001 (India)
- Photobiology Laboratory, Indian Institute of Toxicology Research (CSIR), P.O. Box 80, M.G. Marg, Lucknow 226 001 (India)
- Publication Date:
- OSTI Identifier:
- 22199727
- Resource Type:
- Journal Article
- Journal Name:
- Biochemical and Biophysical Research Communications
- Additional Journal Information:
- Journal Volume: 384; Journal Issue: 2; Other Information: Copyright (c) 2009 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 60 APPLIED LIFE SCIENCES; APOPTOSIS; CARCINOGENESIS; CELL PROLIFERATION; EPITHELIOMAS; IN VITRO; IN VIVO; PHOSPHORYLATION; SERINE; SKIN; TRANSCRIPTION; TRANSCRIPTION FACTORS; TRANSDUCERS; TRANSLOCATION; TYROSINE; ULTRAVIOLET RADIATION
Citation Formats
Roy, Preeti, Kalra, Neetu, Nigam, Nidhi, George, Jasmine, Ray, Ratan Singh, Hans, Rajendra K., Prasad, Sahdeo, and Shukla, Yogeshwer. Resveratrol enhances ultraviolet B-induced cell death through nuclear factor-{kappa}B pathway in human epidermoid carcinoma A431 cells. United States: N. p., 2009.
Web. doi:10.1016/J.BBRC.2009.04.100.
Roy, Preeti, Kalra, Neetu, Nigam, Nidhi, George, Jasmine, Ray, Ratan Singh, Hans, Rajendra K., Prasad, Sahdeo, & Shukla, Yogeshwer. Resveratrol enhances ultraviolet B-induced cell death through nuclear factor-{kappa}B pathway in human epidermoid carcinoma A431 cells. United States. https://doi.org/10.1016/J.BBRC.2009.04.100
Roy, Preeti, Kalra, Neetu, Nigam, Nidhi, George, Jasmine, Ray, Ratan Singh, Hans, Rajendra K., Prasad, Sahdeo, and Shukla, Yogeshwer. 2009.
"Resveratrol enhances ultraviolet B-induced cell death through nuclear factor-{kappa}B pathway in human epidermoid carcinoma A431 cells". United States. https://doi.org/10.1016/J.BBRC.2009.04.100.
@article{osti_22199727,
title = {Resveratrol enhances ultraviolet B-induced cell death through nuclear factor-{kappa}B pathway in human epidermoid carcinoma A431 cells},
author = {Roy, Preeti and Kalra, Neetu and Nigam, Nidhi and George, Jasmine and Ray, Ratan Singh and Hans, Rajendra K. and Prasad, Sahdeo and Shukla, Yogeshwer},
abstractNote = {Resveratrol has been reported to suppress cancer progression in several in vivo and in vitro models, whereas ultraviolet B (UVB), a major risk for skin cancer, is known to induce cell death in cancerous cells. Here, we investigated whether resveratrol can sensitize A431 human epidermoid carcinoma cells to UVB-induced cell death. We examined the combined effect of UVB (30 mJ/cm{sup 2}) and resveratrol (60 {mu}M) on A431 cells. Exposure of A431 carcinoma cells to UVB radiation or resveratrol can inhibit cell proliferation and induce apoptosis. However, the combination of resveratrol and UVB exposure was associated with increased proliferation inhibition of A431 cells compared with either agent alone. Furthermore, results showed that resveratrol and UVB treatment of A431 cells disrupted the nuclear factor-kappaB (NF-{kappa}B) pathway by blocking phosphorylation of serine 536 and inactivating NF-{kappa}B and subsequent degradation of I{kappa}B{alpha}, which regulates the expression of survivin. Resveratrol and UVB treatment also decreased the phosphorylation of tyrosine 701 of the important transcription factor signal transducer activator of transcription (STAT1), which in turn inhibited translocation of phospho-STAT1 to the nucleus. Moreover, resveratrol/UVB also inhibited the metastatic protein LIMK1, which reduced the motility of A431 cells. In conclusion, our study demonstrates that the combination of resveratrol and UVB act synergistically against skin cancer cells. Thus, resveratrol is a potential chemotherapeutic agent against skin carcinogenesis.},
doi = {10.1016/J.BBRC.2009.04.100},
url = {https://www.osti.gov/biblio/22199727},
journal = {Biochemical and Biophysical Research Communications},
issn = {0006-291X},
number = 2,
volume = 384,
place = {United States},
year = {Fri Jun 26 00:00:00 EDT 2009},
month = {Fri Jun 26 00:00:00 EDT 2009}
}