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Title: Oncogenic potential diverge among human papillomavirus type 16 natural variants

Abstract

We compared E6/E7 protein properties of three different HPV-16 variants: AA, E-P and E-350G. Primary human foreskin keratinocytes (PHFK) were transduced with HPV-16 E6 and E7 and evaluated for proliferation and ability to grow in soft agar. E-P infected keratinocytes presented the lowest efficiency in colony formation. AA and E-350G keratinocytes attained higher capacity for in vitro transformation. We observed similar degradation of TP53 among HPV-16 variants. Furthermore, we accessed the expression profile in early (p5) and late passage (p30) transduced cells of 84 genes commonly involved in carcinogenesis. Most differences could be attributed to HPV-16 E6/E7 expression. In particular, we detected different expression of ITGA2 and CHEK2 in keratinocytes infected with AA and AA/E-350G late passage cells, respectively, and higher expression of MAP2K1 in E-350G transduced keratinocytes. Our results indicate differences among HPV-16 variants that could explain, at least in part, differences in oncogenic potential attributed to these variants.

Authors:
 [1];  [1];  [1]
  1. Molecular Biology Laboratory, Center of Translational Oncology, Instituto do Cancer do Estado de Sao Paulo-ICESP, Sao Paulo 01246-000 (Brazil)
Publication Date:
OSTI Identifier:
22150067
Resource Type:
Journal Article
Journal Name:
Virology
Additional Journal Information:
Journal Volume: 432; Journal Issue: 1; Other Information: Copyright (c) 2012 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0042-6822
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; AGAR; CARCINOGENESIS; COLONY FORMATION; GENES; HUMAN POPULATIONS; IN VITRO; PROTEINS; VIRAL DISEASES

Citation Formats

Sichero, Laura, Department of Virology, Ludwig Institute for Cancer Research, Sao Paulo 01323-903, Simao Sobrinho, Joao, Department of Virology, Ludwig Institute for Cancer Research, Sao Paulo 01323-903, Lina Villa, Luisa, Department of Virology, Ludwig Institute for Cancer Research, Sao Paulo 01323-903, and Department of Radiology, School of Medicine, University of Sao Paulo. Oncogenic potential diverge among human papillomavirus type 16 natural variants. United States: N. p., 2012. Web. doi:10.1016/J.VIROL.2012.06.011.
Sichero, Laura, Department of Virology, Ludwig Institute for Cancer Research, Sao Paulo 01323-903, Simao Sobrinho, Joao, Department of Virology, Ludwig Institute for Cancer Research, Sao Paulo 01323-903, Lina Villa, Luisa, Department of Virology, Ludwig Institute for Cancer Research, Sao Paulo 01323-903, & Department of Radiology, School of Medicine, University of Sao Paulo. Oncogenic potential diverge among human papillomavirus type 16 natural variants. United States. https://doi.org/10.1016/J.VIROL.2012.06.011
Sichero, Laura, Department of Virology, Ludwig Institute for Cancer Research, Sao Paulo 01323-903, Simao Sobrinho, Joao, Department of Virology, Ludwig Institute for Cancer Research, Sao Paulo 01323-903, Lina Villa, Luisa, Department of Virology, Ludwig Institute for Cancer Research, Sao Paulo 01323-903, and Department of Radiology, School of Medicine, University of Sao Paulo. 2012. "Oncogenic potential diverge among human papillomavirus type 16 natural variants". United States. https://doi.org/10.1016/J.VIROL.2012.06.011.
@article{osti_22150067,
title = {Oncogenic potential diverge among human papillomavirus type 16 natural variants},
author = {Sichero, Laura and Department of Virology, Ludwig Institute for Cancer Research, Sao Paulo 01323-903 and Simao Sobrinho, Joao and Department of Virology, Ludwig Institute for Cancer Research, Sao Paulo 01323-903 and Lina Villa, Luisa and Department of Virology, Ludwig Institute for Cancer Research, Sao Paulo 01323-903 and Department of Radiology, School of Medicine, University of Sao Paulo},
abstractNote = {We compared E6/E7 protein properties of three different HPV-16 variants: AA, E-P and E-350G. Primary human foreskin keratinocytes (PHFK) were transduced with HPV-16 E6 and E7 and evaluated for proliferation and ability to grow in soft agar. E-P infected keratinocytes presented the lowest efficiency in colony formation. AA and E-350G keratinocytes attained higher capacity for in vitro transformation. We observed similar degradation of TP53 among HPV-16 variants. Furthermore, we accessed the expression profile in early (p5) and late passage (p30) transduced cells of 84 genes commonly involved in carcinogenesis. Most differences could be attributed to HPV-16 E6/E7 expression. In particular, we detected different expression of ITGA2 and CHEK2 in keratinocytes infected with AA and AA/E-350G late passage cells, respectively, and higher expression of MAP2K1 in E-350G transduced keratinocytes. Our results indicate differences among HPV-16 variants that could explain, at least in part, differences in oncogenic potential attributed to these variants.},
doi = {10.1016/J.VIROL.2012.06.011},
url = {https://www.osti.gov/biblio/22150067}, journal = {Virology},
issn = {0042-6822},
number = 1,
volume = 432,
place = {United States},
year = {Wed Oct 10 00:00:00 EDT 2012},
month = {Wed Oct 10 00:00:00 EDT 2012}
}