Induction of IL-6 and inhibition of IL-8 secretion in the human airway cell line Calu-3 by urban particulate matter collected with a modified method of PM sampling
- Lung Toxicology Unit, Pneumology Section, K.U. Leuven (Belgium)
- Departamento Farmacologia, Facultad de Medicina, U.N.A.M. (Mexico)
- Departamento de Fisica Experimental, Instituto de Fisca, U.N.A.M. (Mexico)
- Deparatmento de Aerobiologia, Centro de Ciencias de la Atmosfera - Facultad de Medicina, U.N.A.M. (Mexico)
- Subdireccion de Investigacion Basica, Instituto Nacional de Cancerologia, Avenida San Fernando 22, C.P. 14080, Mexico D.F. (Mexico)
- Escuela Superior de Ingenieria Quimica e Industrias Extractivas, I.P.N. (Mexico)
Exposure to particulate matter (PM) induces inflammatory cytokines. In the present study, we evaluated the secretion of IL-6 and IL-8 by an airway cell line exposed to PM with a mean aerodynamic size equal to or less than 10 or 2.5 {mu}m (PM{sub 10} and PM{sub 2.5}, respectively) collected in Mexico City, using a modified high-volume sampling method avoiding the use of solvents or introducing membrane components into the samples. PM was collected on cellulose-nitrate (CN) membranes modified for collection on high-volume samplers. Composition of the particles was evaluated by particle-induced X-ray emission (PIXE) and scanning electron microscopy. The particles (10-160 {mu}g/cm{sup 2}) were tested on Calu-3 cells. Control cultures were exposed to LPS (10 ng/mL to 100 {mu}g/mL) or silica (10-160 {mu}g/cm{sup 2}). IL-6 and IL-8 secretions were evaluated by ELISA. An average of 10 mg of PM was recovered form each cellulose-nitrate filter. No evidence of contamination from the filter was found. Cells exposed to PM{sub 10} presented an increase in the secretion of IL-6 (up to 400%), while IL-8 decreased (from 40% to levels below the detection limit). A similar but weaker effect was observed with PM{sub 2.5}. In conclusion, our modified sampling method provides a large amount of urban PM free of membrane contamination. The urban particles induce a decrease in IL-8 secretion that contrasts with the LPS and silica effects. These results suggest that the regulation of IL-8 expression is different for urban particles (complex mixture containing combustion-related particles, soil and biologic components) than for biogenic compounds or pure mineral particles.
- OSTI ID:
- 22149197
- Journal Information:
- Environmental Research, Vol. 109, Issue 5; Other Information: Copyright (c) 2009 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); ISSN 0013-9351
- Country of Publication:
- United States
- Language:
- English
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