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Title: Laser-induced fusion of human embryonic stem cells with optical tweezers

We report a study on the laser-induced fusion of human embryonic stem cells (hESCs) at the single-cell level. Cells were manipulated by optical tweezers and fused under irradiation with pulsed UV laser at 355 nm. Successful fusion was indicated by green fluorescence protein transfer. The influence of laser pulse energy on the fusion efficiency was investigated. The fused products were viable as gauged by live cell staining. Successful fusion of hESCs with somatic cells was also demonstrated. The reported fusion outcome may facilitate studies of cell differentiation, maturation, and reprogramming.
Authors:
; ;  [1] ; ;  [2] ;  [3] ;  [3] ;  [4]
  1. Department of Mechanical and Biomedical Engineering, City University of Hong Kong (Hong Kong)
  2. Department of Biology and Chemistry, City University of Hong Kong (Hong Kong)
  3. Stem Cell and Regenerative Medicine Consortium, and Departments of Medicine and Physiology, LKS Faculty of Medicine, University of Hong Kong (Hong Kong)
  4. (United States)
Publication Date:
OSTI Identifier:
22122827
Resource Type:
Journal Article
Resource Relation:
Journal Name: Applied Physics Letters; Journal Volume: 103; Journal Issue: 3; Other Information: (c) 2013 AIP Publishing LLC; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
71 CLASSICAL AND QUANTUM MECHANICS, GENERAL PHYSICS; BIOLOGICAL EFFECTS; BIOPHYSICS; CELL DIFFERENTIATION; EFFICIENCY; FLUORESCENCE; IRRADIATION; LASER RADIATION; PROTEINS; PULSES; RADIATION PRESSURE; STEM CELLS