Radiosensitizing effect of gold nanoparticles in carbon ion irradiation of human cervical cancer cells

Journal Article · Thu Jul 18 00:00:00 EDT 2013 · AIP Conference Proceedings

DOI:https://doi.org/10.1063/1.4812924· OSTI ID:22121666

Kaur, Harminder; Avasthi, D. K.; Pujari, Geetanjali; Sarma, Asitikantha ^[1]

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1. Inter University Accelerator Centre, Aruna Asaf Ali Marg, Post box-10502, New Delhi-110067 (India)

Noble metal nanoparticles have received considerable attention in biotechnology for their role in bio sensing due to surface plasmon resonance, medical diagnostics due to better imaging contrast and therapy. The radiosensitization effect of gold nanoparticles (AuNP) has been gaining popularity in radiation therapy of cancer cells. The better depth dose profile of energetic ion beam proves its superiority over gamma radiation for fighting against cancer. In the present work, the glucose capped gold nanoparticles (Glu-AuNP) were synthesised and internalized in the HeLa cells. Transmission electron microscopic analysis of ultrathin sections of Glu-AuNP treated HeLa cells confirmed the internalization of Glu-AuNPs. Control HeLa cells and Glu-AuNp treated HeLa cells were irradiated at different doses of 62 MeV 12C ion beam (LET - 290keV/{mu}m) at BIO beam line of using 15UD Pelletron accelerator at Inter University Accelerator Centre, New Delhi, India. The survival fraction was assessed by colony forming assay which revealed that the dose of carbon ion for 90% cell killing in Glu-AuNP treated HeLa cells and control HeLa cells are 2.3 and 3.2 Gy respectively. This observation shows {approx} 28% reduction of {sup 12}C{sup 6+} ion dose for Glu-AuNP treated HeLa cells as compared to control HeLa cells.

• OSTI ID: 22121666
• Journal Information: AIP Conference Proceedings, Vol. 1530, Issue 1; Conference: ION BEAMS '12: International conference on multidisciplinary applications of nuclear physics with ion beams, Legnaro, Padova (Italy), 6-8 Jun 2012; Other Information: (c) 2013 AIP Publishing LLC; Country of input: International Atomic Energy Agency (IAEA); ISSN 0094-243X
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS
61 RADIATION PROTECTION AND DOSIMETRY
BIOTECHNOLOGY
CARBON 12
CARBON IONS
CELL KILLING
DEPTH DOSE DISTRIBUTIONS
GAMMA RADIATION
GLUCOSE
GOLD
HELA CELLS
ION BEAMS
MEV RANGE
NANOSTRUCTURES
PELLETRON ACCELERATORS
PLASMONS
RADIATION EFFECTS
RADIOTHERAPY
SACCHAROSE
SURVIVAL CURVES
TAIL IONS
TRANSMISSION ELECTRON MICROSCOPY