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Title: Noncontact microsurgery of cell membranes using femtosecond laser pulses for optoinjection of specified substances into cells

Abstract

IR femtosecond laser pulses were used for microsurgery of a cell membrane aimed at local and short-duration change in its permeability and injection of specified extracellular substances into the cells. The possibility of noncontact laser delivery of the propidium iodide fluorescent dye and the pEGFP plasmid, encoding the green fluorescent protein, into the cells with preservation of the cell viability was demonstrated. (extreme light fields and their applications)

Authors:
; ; ; ; ;
Publication Date:
OSTI Identifier:
22113694
Resource Type:
Journal Article
Journal Name:
Quantum Electronics (Woodbury, N.Y.)
Additional Journal Information:
Journal Volume: 43; Journal Issue: 4; Other Information: Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 1063-7818
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; CELL MEMBRANES; FLUORESCENCE; INFRARED RADIATION; IODIDES; LASER RADIATION; PROTEINS; PULSES; VIABILITY

Citation Formats

Il'ina, I V, Ovchinnikov, A V, Chefonov, O V, Sitnikov, D S, Agranat, Mikhail B, and Mikaelyan, A S. Noncontact microsurgery of cell membranes using femtosecond laser pulses for optoinjection of specified substances into cells. United States: N. p., 2013. Web. doi:10.1070/QE2013V043N04ABEH015115.
Il'ina, I V, Ovchinnikov, A V, Chefonov, O V, Sitnikov, D S, Agranat, Mikhail B, & Mikaelyan, A S. Noncontact microsurgery of cell membranes using femtosecond laser pulses for optoinjection of specified substances into cells. United States. https://doi.org/10.1070/QE2013V043N04ABEH015115
Il'ina, I V, Ovchinnikov, A V, Chefonov, O V, Sitnikov, D S, Agranat, Mikhail B, and Mikaelyan, A S. 2013. "Noncontact microsurgery of cell membranes using femtosecond laser pulses for optoinjection of specified substances into cells". United States. https://doi.org/10.1070/QE2013V043N04ABEH015115.
@article{osti_22113694,
title = {Noncontact microsurgery of cell membranes using femtosecond laser pulses for optoinjection of specified substances into cells},
author = {Il'ina, I V and Ovchinnikov, A V and Chefonov, O V and Sitnikov, D S and Agranat, Mikhail B and Mikaelyan, A S},
abstractNote = {IR femtosecond laser pulses were used for microsurgery of a cell membrane aimed at local and short-duration change in its permeability and injection of specified extracellular substances into the cells. The possibility of noncontact laser delivery of the propidium iodide fluorescent dye and the pEGFP plasmid, encoding the green fluorescent protein, into the cells with preservation of the cell viability was demonstrated. (extreme light fields and their applications)},
doi = {10.1070/QE2013V043N04ABEH015115},
url = {https://www.osti.gov/biblio/22113694}, journal = {Quantum Electronics (Woodbury, N.Y.)},
issn = {1063-7818},
number = 4,
volume = 43,
place = {United States},
year = {Tue Apr 30 00:00:00 EDT 2013},
month = {Tue Apr 30 00:00:00 EDT 2013}
}