Atomic force microscopy study of the adsorption of protein molecules on transferred Langmuir monolayer

Journal Article · Wed Sep 15 00:00:00 EDT 2010 · Crystallography Reports

DOI:https://doi.org/10.1134/S1063774510050251· OSTI ID:22054086

Tolstikhina, A L; Stepina, N D ^[1]; Novikova, N N ^[2]; Yur'eva, E A ^[1]; Khripunov, A K ^[3]

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1. Russian Academy of Sciences, Shubnikov Institute of Crystallography (Russian Federation)
2. Russian Research Center Kurchatov Institute (Russian Federation)
3. Russian Academy of Sciences, Institute of Macromolecular Compounds (Russian Federation)

Ordered protein films have been obtained by the adsorption of protein molecules on a Langmuir monolayer, which had previously formed on a silicon substrate, using the Langmuir-Blodgett and molecular self-organization methods. A mixture of cholesterol with dipalmitoylphosphatidylcholine (DPPC) and a polymer-cellulose acetopivalinate-were used as immobilization materials. Protein molecules (catalase and alkaline phosphatase) immobilized on solid substrates have been investigated by atomic force micros-copy. It was shown that the developed combined technique provides a deposition of homogeneous ultrathin protein films with a high degree of filling.

• OSTI ID: 22054086
• Journal Information: Crystallography Reports, Vol. 55, Issue 5; Other Information: Copyright (c) 2010 Pleiades Publishing, Ltd.; Country of input: International Atomic Energy Agency (IAEA); ISSN 1063-7745
• Country of Publication: United States
• Language: English

Related Subjects

75 CONDENSED MATTER PHYSICS
SUPERCONDUCTIVITY AND SUPERFLUIDITY
ADSORPTION
ALKALINE PHOSPHATASE
ATOMIC FORCE MICROSCOPY
CATALASE
CELLULOSE
CHOLESTEROL
FILMS
POLYMERS
SILICON