Neural induced embryoid bodies present high levels of metals detected by x-ray microfluorescence
- Instituto de Ciencias Biomedicas, Universidade Federal do Rio de Janeiro, Av. Carlos Chagas, 373 (Brazil)
Molecular mechanisms driving neural differentiation in human embryonic stem cells are not completely elucidated, specially, the role of atomic elements within this process. In this work, we described the distribution of trace elements in those stem cells growing as embryoid bodies by using synchrotron radiation X-ray microfluorescence (SR-XRF). Naive and neural induced embryoid bodies derived from embryonic stem cells were irradiated with a spatial resolution of 20 {mu}m to make elemental maps and qualitative chemical analyses. We consistently detected metallic elements content raise on neural induced embryoid bodies, mimicking characteristic brain development. The use of SR-XRF reveals that human embryoid bodies exhibit self-organization at the atomic level, which is enhanced during neurogenesis triggered in vitro.
- OSTI ID:
- 22004048
- Journal Information:
- AIP Conference Proceedings, Vol. 1437, Issue 1; Conference: 21. international congress on X-ray optics and microanalysis, Campinas (Brazil), 5-9 Sep 2011; Other Information: (c) 2012 American Institute of Physics; Country of input: International Atomic Energy Agency (IAEA); ISSN 0094-243X
- Country of Publication:
- United States
- Language:
- English
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