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Title: 3D Chemical and Elemental Imaging by STXM Spectrotomography

Spectrotomography based on the scanning transmission x-ray microscope (STXM) at the 10ID-1 spectromicroscopy beamline of the Canadian Light Source was used to study two selected unicellular microorganisms. Spatial distributions of sulphur globules, calcium, protein, and polysaccharide in sulphur-metabolizing bacteria (Allochromatium vinosum) were determined at the S 2p, C 1s, and Ca 2p edges. 3D chemical mapping showed that the sulphur globules are located inside the bacteria with a strong spatial correlation with calcium ions (it is most probably calcium carbonate from the medium; however, with STXM the distribution and localization in the cell can be made visible, which is very interesting for a biologist) and polysaccharide-rich polymers, suggesting an influence of the organic components on the formation of the sulphur and calcium deposits. A second study investigated copper accumulating in yeast cells (Saccharomyces cerevisiae) treated with copper sulphate. 3D elemental imaging at the Cu 2p edge showed that Cu(II) is reduced to Cu(I) on the yeast cell wall. A novel needle-like wet cell sample holder for STXM spectrotomography studies of fully hydrated samples is discussed.
Authors:
; ; ;  [1] ;  [2] ;  [3] ;  [4] ;  [5] ;  [3] ;  [6] ;  [7]
  1. Canadian Light Source Inc., University of Saskatchewan, Saskatoon, SK S7N 0X4 (Canada)
  2. BIMR, McMaster University, Hamilton, ON L8S 4M1 (Canada)
  3. Microbiology and Food Hygiene, Niederrhein University of Applied Sciences, Moenchengladbach (Germany)
  4. (Germany)
  5. (CAMD), Louisiana State University, Baton Rouge, LA (United States)
  6. College of Medicine, University of Saskatchewan, Saskatoon, SK S7N 5E5 (Canada)
  7. Center for Applied Geoscience, Tuebingen University, Tuebingen (Germany)
Publication Date:
OSTI Identifier:
21608285
Resource Type:
Journal Article
Resource Relation:
Journal Name: AIP Conference Proceedings; Journal Volume: 1365; Journal Issue: 1; Conference: 10. international conference on X-ray microscopy, Chicago, IL (United States), 15-20 Aug 2010; Other Information: DOI: 10.1063/1.3625342; (c) 2011 American Institute of Physics; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; 43 PARTICLE ACCELERATORS; ABSORPTION SPECTROSCOPY; BACTERIA; CALCIUM; CALCIUM CARBONATES; CALCIUM IONS; CELL WALL; COPPER; IMAGE PROCESSING; IMAGES; LIGHT SOURCES; MAPPING; POLYSACCHARIDES; PROTEINS; SACCHAROMYCES CEREVISIAE; SPATIAL DISTRIBUTION; SULFATES; SULFUR; X RADIATION; X-RAY SPECTROSCOPY ALKALINE EARTH METAL COMPOUNDS; ALKALINE EARTH METALS; CALCIUM COMPOUNDS; CARBOHYDRATES; CARBON COMPOUNDS; CARBONATES; CELL CONSTITUENTS; CHARGED PARTICLES; DISTRIBUTION; ELECTROMAGNETIC RADIATION; ELEMENTS; EUMYCOTA; FUNGI; IONIZING RADIATIONS; IONS; METALS; MICROORGANISMS; NONMETALS; ORGANIC COMPOUNDS; OXYGEN COMPOUNDS; PLANTS; PROCESSING; RADIATION SOURCES; RADIATIONS; SACCHARIDES; SACCHAROMYCES; SPECTROSCOPY; SULFUR COMPOUNDS; TRANSITION ELEMENTS; YEASTS