3D Chemical and Elemental Imaging by STXM Spectrotomography

Journal Article · Fri Sep 09 00:00:00 EDT 2011 · AIP Conference Proceedings

DOI:https://doi.org/10.1063/1.3625342· OSTI ID:21608285

Wang, J; Karunakaran, C; Lu, Y; Hormes, J ^[1]; Hitchcock, A P ^[2]; Prange, A ^[3]; Franz, B ^[3]; Harkness, T ^[4]; Obst, M ^[5]

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1. Canadian Light Source Inc., University of Saskatchewan, Saskatoon, SK S7N 0X4 (Canada)
2. BIMR, McMaster University, Hamilton, ON L8S 4M1 (Canada)
3. Microbiology and Food Hygiene, Niederrhein University of Applied Sciences, Moenchengladbach (Germany)
4. College of Medicine, University of Saskatchewan, Saskatoon, SK S7N 5E5 (Canada)
5. Center for Applied Geoscience, Tuebingen University, Tuebingen (Germany)

Spectrotomography based on the scanning transmission x-ray microscope (STXM) at the 10ID-1 spectromicroscopy beamline of the Canadian Light Source was used to study two selected unicellular microorganisms. Spatial distributions of sulphur globules, calcium, protein, and polysaccharide in sulphur-metabolizing bacteria (Allochromatium vinosum) were determined at the S 2p, C 1s, and Ca 2p edges. 3D chemical mapping showed that the sulphur globules are located inside the bacteria with a strong spatial correlation with calcium ions (it is most probably calcium carbonate from the medium; however, with STXM the distribution and localization in the cell can be made visible, which is very interesting for a biologist) and polysaccharide-rich polymers, suggesting an influence of the organic components on the formation of the sulphur and calcium deposits. A second study investigated copper accumulating in yeast cells (Saccharomyces cerevisiae) treated with copper sulphate. 3D elemental imaging at the Cu 2p edge showed that Cu(II) is reduced to Cu(I) on the yeast cell wall. A novel needle-like wet cell sample holder for STXM spectrotomography studies of fully hydrated samples is discussed.

• OSTI ID: 21608285
• Journal Information: AIP Conference Proceedings, Vol. 1365, Issue 1; Conference: 10. international conference on X-ray microscopy, Chicago, IL (United States), 15-20 Aug 2010; Other Information: DOI: 10.1063/1.3625342; (c) 2011 American Institute of Physics; Country of input: International Atomic Energy Agency (IAEA); ISSN 0094-243X
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
43 PARTICLE ACCELERATORS
ABSORPTION SPECTROSCOPY
BACTERIA
CALCIUM
CALCIUM CARBONATES
CALCIUM IONS
CELL WALL
COPPER
IMAGE PROCESSING
IMAGES
LIGHT SOURCES
MAPPING
POLYSACCHARIDES
PROTEINS
SACCHAROMYCES CEREVISIAE
SPATIAL DISTRIBUTION
SULFATES
SULFUR
X RADIATION
X-RAY SPECTROSCOPY
ALKALINE EARTH METAL COMPOUNDS
ALKALINE EARTH METALS
CALCIUM COMPOUNDS
CARBOHYDRATES
CARBON COMPOUNDS
CARBONATES
CELL CONSTITUENTS
CHARGED PARTICLES
DISTRIBUTION
ELECTROMAGNETIC RADIATION
ELEMENTS
EUMYCOTA
FUNGI
IONIZING RADIATIONS
IONS
METALS
MICROORGANISMS
NONMETALS
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
PLANTS
PROCESSING
RADIATION SOURCES
RADIATIONS
SACCHARIDES
SACCHAROMYCES
SPECTROSCOPY
SULFUR COMPOUNDS
TRANSITION ELEMENTS
YEASTS