Baicalein inhibits the migration and invasive properties of human hepatoma cells
- Emergency Department and Center of Hyperbaric Oxygen Therapy, Tungs' Taichung MetroHarbor Hospital, Taichung, Taiwan (China)
- Division of Hematology, Department of Internal Medicine, Taichung Veterans General Hospital, Taichung, Taiwan (China)
- Division of Colon and Rectal Surgery, Department of Surgery, Chang Gung Memorial Hospital Chiayi, Taiwan (China)
- Division of Gastroenterology, Department of Internal Medicine, Armed-Forces Taichung General Hospital, Taiping City, Taichung, Taiwan (China)
- Department of Public Health, National Defense Medical Center, Taipei, Taiwan (China)
- Department of Internal Medicine, Armed-Forces Taichung General Hospital, Taiwan (China)
- Department of Pathology, Chung Shan Medical University and Hospital, Taichung, Taiwan (China)
- Department of Medical Technology, Central Taiwan University of Science and Technology, Taichung, Taiwan (China)
- Department of Surgery, Tungs' Taichung MetroHarbor Hospital, Taichung, Taiwan (China)
- Graduate Institute of Basic Medical Science, China Medical University, Taiwan (China)
- Graduate Institute of Cancer Biology, College of Medicine, China Medical University, Taichung, Taiwan (China)
- Department of Food and Nutrition, Taichung Veterans General Hospital, Taichung, Taiwan (China)
- School of Applied Chemistry, Chung Shan Medical University, Taichung, Taiwan (China)
Flavonoids have been demonstrated to exert health benefits in humans. We investigated whether the flavonoid baicalein would inhibit the adhesion, migration, invasion, and growth of human hepatoma cell lines, and we also investigated its mechanism of action. The separate effects of baicalein and baicalin on the viability of HA22T/VGH and SK-Hep1 cells were investigated for 24 h. To evaluate their invasive properties, cells were incubated on matrigel-coated transwell membranes in the presence or absence of baicalein. We examined the effect of baicalein on the adhesion of cells, on the activation of matrix metalloproteinases (MMPs), protein kinase C (PKC), and p38 mitogen-activated protein kinase (MAPK), and on tumor growth in vivo. We observed that baicalein suppresses hepatoma cell growth by 55%, baicalein-treated cells showed lower levels of migration than untreated cells, and cell invasion was significantly reduced to 28%. Incubation of hepatoma cells with baicalein also significantly inhibited cell adhesion to matrigel, collagen I, and gelatin-coated substrate. Baicalein also decreased the gelatinolytic activities of the matrix metalloproteinases MMP-2, MMP-9, and uPA, decreased p50 and p65 nuclear translocation, and decreased phosphorylated I-kappa-B (IKB)-{beta}. In addition, baicalein reduced the phosphorylation levels of PKC{alpha} and p38 proteins, which regulate invasion in poorly differentiated hepatoma cells. Finally, when SK-Hep1 cells were grown as xenografts in nude mice, intraperitoneal (i.p.) injection of baicalein induced a significant dose-dependent decrease in tumor growth. These results demonstrate the anticancer properties of baicalein, which include the inhibition of adhesion, invasion, migration, and proliferation of human hepatoma cells in vivo. - Highlight: > Baicalein inhibits several essential steps in the onset of metastasis.
- OSTI ID:
- 21587837
- Journal Information:
- Toxicology and Applied Pharmacology, Vol. 255, Issue 3; Other Information: DOI: 10.1016/j.taap.2011.07.008; PII: S0041-008X(11)00268-7; Copyright (c) 2011 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); ISSN 0041-008X
- Country of Publication:
- United States
- Language:
- English
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