Metabolites of arsenic and increased DNA damage of p53 gene in arsenic plant workers
- Guizhou College of Finance and Economics, No. 276, Chongguan Road, Guiyang, Guizhou, 550004 (China)
- Department of Occupational Health, Yunnan Provincial Center for Disease Control and Prevention, No. 158, Dongsi Street, Kunming, Yunnan, 650022 (China)
- The First Affiliated Hospital of Kunming Medical College, No. 295 Xichang Road, Kunming, Yunnan, 650032 (China)
- The First Division of Public Health, Kunming Center for Disease Control and Prevention, No. 4, Ziyun Road, Xishan District, Kunming, Yunnan 650228 (China)
- Honghe Zhou Center for Disease Control and Prevention, No. 1, Guannan Road, Mengzi City, Yunnan, 661100 (China)
- Yunnan Institute for Drug Abuse, Kunming, 650028 (China)
Recent studies have shown that monomethylarsonous acid is more cytotoxic and genotoxic than arsenate and arsenite, which may attribute to the increased levels of reactive oxygen species. In this study, we used hydride generation-atomic absorption spectrometry to determine three arsenic species in urine of workers who had been working in arsenic plants,and calculated primary and secondary methylation indexes. The damages of exon 5, 6, 8 of p53 gene were determined by the method developed by Sikorsky, et al. Results show that the concentrations of each urinary arsenic species,and damage indexes of exon 5 and 8 of p53 gene in the exposed population were significantly higher, but SMI was significantly lower than in the control group. The closely positive correlation between the damage index of exon 5 and PMI,MMA, DMA were found, but there was closely negative correlation between the damage index of exon 5 and SMI. Those findings suggested that DNA damage of exon 5 and 8 of p53 gene existed in the population occupationally exposed to arsenic. For exon 5, the important factors may include the model of arsenic metabolic transformation, the concentrations of MMA and DMA, and the MMA may be of great importance. - Research Highlights: > In our study, the mean SMI for workers came from arsenic plants is 4.06, so they may be in danger. > There are more MMA, there are more damage of exon 5 of p53 gene. > MMA and damage of exon 5 of p53 gene may be useful biomarkers to assess adverse health effects caused by arsenic.
- OSTI ID:
- 21587776
- Journal Information:
- Toxicology and Applied Pharmacology, Vol. 254, Issue 1; Other Information: DOI: 10.1016/j.taap.2011.04.013; PII: S0041-008X(11)00152-9; Copyright (c) 2011 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); ISSN 0041-008X
- Country of Publication:
- United States
- Language:
- English
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