A study of the enhanced sensitizing capacity of a contact allergen in lipid vesicle formulations

Journal Article · Sun May 01 00:00:00 EDT 2011 · Toxicology and Applied Pharmacology

DOI:https://doi.org/10.1016/j.taap.2011.02.010· OSTI ID:21535292

Simonsson, Carl ^[1]; Madsen, Jakob Torp ^[2]; Graneli, Annette ^[3]; Andersen, Klaus E ^[2]; Karlberg, Ann-Therese; Jonsson, Charlotte A ^[1]; Ericson, Marica B ^[3]

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1. Department of Chemistry, University of Gothenburg, SE-412 96, Gothenburg (Sweden)
2. Department of Dermatology, Odense University Hospital, University of Southern Denmark, DK-5000, Odense (Denmark)
3. Department of Physics, University of Gothenburg, SE-412 96, Gothenburg (Sweden)

The growing focus on nanotechnology and the increased use of nano-sized structures, e.g. vesicles, in topical formulations has led to safety concerns. We have investigated the sensitizing capacity and penetration properties of a fluorescent model compound, rhodamine B isothiocyanate (RBITC), when administered in micro- and nano-scale vesicle formulations. The sensitizing capacity of RBITC was studied using the murine local lymph node assay (LLNA) and the skin penetration properties were compared using diffusion cells in combination with two-photon microscopy (TPM). The lymph node cell proliferation, an indicator of a compounds sensitizing capacity, increased when RBITC was applied in lipid vesicles as compared to an ethanol:water (Et:W) solution. Micro-scale vesicles showed a slightly higher cell proliferative response compared to nano-scale vesicles. TPM imaging revealed that the vesicle formulations improved the skin penetration of RBITC compared to the Et:W solution. A strong fluorescent region in the stratum corneum and upper epidermis implies elevated association of RBITC to these skin layers when formulated in lipid vesicles. In conclusion, the results indicate that there could be an elevated risk of sensitization when haptens are delivered in vehicles containing lipid vesicles. Although the size of the vesicles seems to be of minor importance, further studies are needed before a more generalized conclusion can be drawn. It is likely that the enhanced sensitizing capacity is a consequence of the improved penetration and increased formation of hapten-protein complexes in epidermis when RBITC is delivered in ethosomal formulations. - Graphical Abstract: Display Omitted

• OSTI ID: 21535292
• Journal Information: Toxicology and Applied Pharmacology, Vol. 252, Issue 3; Other Information: DOI: 10.1016/j.taap.2011.02.010; PII: S0041-008X(11)00056-1; Copyright (c) 2011 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; ISSN 0041-008X
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
CAPACITY
CELL PROLIFERATION
COMPLEXES
DERMATITIS
EPIDERMIS
FLUORESCENCE
HAZARDS
ISOTHIOCYANATES
LIPIDS
LYMPH NODES
MICROSCOPY
NANOSTRUCTURES
SAFETY
SOLUTIONS
ANIMAL TISSUES
BODY
CARBONIC ACID DERIVATIVES
DISEASES
DISPERSIONS
EMISSION
EPITHELIUM
HOMOGENEOUS MIXTURES
LUMINESCENCE
LYMPHATIC SYSTEM
MIXTURES
NITROGEN COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
PHOTON EMISSION
SKIN
SKIN DISEASES