Synthesis, Characterization, and Preliminary Investigation of Cell Interaction of Magnetic Nanoparticles with Catechol-Containing Shells

Journal Article · Thu Dec 02 00:00:00 EST 2010 · AIP Conference Proceedings

DOI:https://doi.org/10.1063/1.3530028· OSTI ID:21506933

Wagner, Kerstin; Seemann, Thomas; Wyrwa, Ralf; Schnabelrauch, Matthias ^[1]; Clement, Joachim H ^[2]; Mueller, Robert ^[3]; Nietzsche, Sandor ^[4]

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1. Biomaterials Department, INNOVENT e. V., Pruessingstrasse 27 B, D-07745 Jena (Germany)
2. Department of Internal Medicine II, University Hospital Jena, Erlanger Allee 101, D-07740 Jena (Germany)
3. Institute of Photonic Technology, Albert-Einstein-Strasse 9, D-07745 Jena (Germany)
4. Center for Electron Microscopy, University Hospital Jena, Ziegelmuehlenweg 1, D-07743 Jena (Germany)

Superparamagnetic iron oxide cores were synthesized by co-precipitation of Fe(II) and Fe(III) salts and subsequently stabilized by coating with different catechols (levodopa, dopamine, hydrocaffeic acid, dopamine-containing carboxymethyl dextran) known to act as high-affinity, bidentate ligands for Fe(III). The prepared stable magnetic fluids were characterized with regard to their chemical composition (content of iron and shell material, Fe(II)/Fe(III) ratio) and their physical properties (size, surface charge, magnetic parameters). The nanoparticles showed no or only slight cytotoxic effects within 1 and 4 days of incubation with 3T3 fibroblast cells. Preliminary experiments were performed to study the interaction of the prepared nanoparticles with human MCF-7 breast cancer cells and leukocytes. An intense interaction of the MCF-7 cells with these particles was found whereas the leukocytes showed a lower tendency of interaction. Based on these finding, the novel magnetic nanoparticles possess the potential for use in depletion of tumor cells from peripheral blood.

• OSTI ID: 21506933
• Journal Information: AIP Conference Proceedings, Vol. 1311, Issue 1; Conference: 8. international conference on the scientific and clinical applications of magnetic carriers, Rostock (Germany), 25-29 May 2010; Other Information: DOI: 10.1063/1.3530028; (c) 2010 American Institute of Physics; ISSN 0094-243X
• Country of Publication: United States
• Language: English

Related Subjects

77 NANOSCIENCE AND NANOTECHNOLOGY
CHEMICAL ANALYSIS
CHEMICAL COMPOSITION
COPRECIPITATION
DEXTRAN
DOPAMINE
FIBROBLASTS
IRON COMPOUNDS
IRON OXIDES
LEUKOCYTES
MAMMARY GLANDS
NANOSTRUCTURES
NEOPLASMS
PARTICLES
PHYSICAL PROPERTIES
PYROCATECHOL
SALTS
SUPERPARAMAGNETISM
SURFACES
SYNTHESIS
TUMOR CELLS
AMINES
ANIMAL CELLS
AROMATICS
AUTONOMIC NERVOUS SYSTEM AGENTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD SUBSTITUTES
BODY
BODY FLUIDS
CARBOHYDRATES
CARDIOTONICS
CARDIOVASCULAR AGENTS
CHALCOGENIDES
CONNECTIVE TISSUE CELLS
DEVELOPERS
DISEASES
DRUGS
GLANDS
HEMATOLOGIC AGENTS
HYDROXY COMPOUNDS
MAGNETISM
MATERIALS
NEUROREGULATORS
ORGANIC COMPOUNDS
ORGANS
OXIDES
OXYGEN COMPOUNDS
PHENOLS
POLYPHENOLS
POLYSACCHARIDES
PRECIPITATION
SACCHARIDES
SEPARATION PROCESSES
SOMATIC CELLS
SYMPATHOMIMETICS
TRANSITION ELEMENT COMPOUNDS