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Title: Four-photon microwave laser spectroscopy of aqueous solutions of biopolymers

Abstract

The four-photon laser radiation scattering spectra are obtained in the submillimetre range (75-95 cm{sup -1}) for deionised water, aqueous solutions of DNA and {alpha}-chymotrypsin protein. Narrow resonances are recorded whose frequencies coincide (within the resolution power of a spectrometer) with rotational frequencies in the ground electronic state and vibrational state of ortho and para isomers of H{sub 2}O molecule in a gas phase and with the frequencies of the lines of H{sub 2}O{sub 2} and OH{sup -} molecules. It is shown that the resonance contribution of the rotational lines of ortho isomers of H{sub 2}O to the signal of four-photon scattering of native solutions of biopolymers increases by a factor of at least 8 compared to their contribution to the scattering signal in water, and becomes considerably larger than the contribution from the paraisomer lines. Denaturation of DNA after heating and cooling of the solution leads to the disappearance of such selectivity. (special issue devoted to the 25th anniversary of the a.m. prokhorov general physics institute)

Authors:
; ;  [1];  [2];  [3]
  1. A.M. Prokhorov General Physics Institute, Russian Academy of Sciences, Moscow (Russian Federation)
  2. Institute for Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Moscow region (Russian Federation)
  3. Institute of Protein Research, RAS, Pushchino, Moscow region (Russian Federation)
Publication Date:
OSTI Identifier:
21466736
Resource Type:
Journal Article
Journal Name:
Quantum Electronics (Woodbury, N.Y.)
Additional Journal Information:
Journal Volume: 37; Journal Issue: 10; Other Information: DOI: 10.1070/QE2007v037n10ABEH013609; Journal ID: ISSN 1063-7818
Country of Publication:
United States
Language:
English
Subject:
74 ATOMIC AND MOLECULAR PHYSICS; AQUEOUS SOLUTIONS; CHYMOTRYPSIN; COOLING; DNA; HEATING; HYDROGEN PEROXIDE; HYDROXIDES; LASER RADIATION; LASER SPECTROSCOPY; MICROWAVE RADIATION; MOLECULES; PHOTONS; RESOLUTION; RESONANCE; SCATTERING; SIGNALS; SPECTRA; SPECTROMETERS; BOSONS; DISPERSIONS; ELECTROMAGNETIC RADIATION; ELEMENTARY PARTICLES; ENZYMES; HOMOGENEOUS MIXTURES; HYDROGEN COMPOUNDS; HYDROLASES; MASSLESS PARTICLES; MEASURING INSTRUMENTS; MIXTURES; NUCLEIC ACIDS; ORGANIC COMPOUNDS; OXYGEN COMPOUNDS; PEPTIDE HYDROLASES; PEROXIDES; PROTEINS; RADIATIONS; SERINE PROTEINASES; SOLUTIONS; SPECTROSCOPY

Citation Formats

Bunkin, A F, Nurmatov, A A, Pershin, S M, Khusainova, R S, and Potekhin, S A. Four-photon microwave laser spectroscopy of aqueous solutions of biopolymers. United States: N. p., 2007. Web. doi:10.1070/QE2007V037N10ABEH013609.
Bunkin, A F, Nurmatov, A A, Pershin, S M, Khusainova, R S, & Potekhin, S A. Four-photon microwave laser spectroscopy of aqueous solutions of biopolymers. United States. https://doi.org/10.1070/QE2007V037N10ABEH013609
Bunkin, A F, Nurmatov, A A, Pershin, S M, Khusainova, R S, and Potekhin, S A. 2007. "Four-photon microwave laser spectroscopy of aqueous solutions of biopolymers". United States. https://doi.org/10.1070/QE2007V037N10ABEH013609.
@article{osti_21466736,
title = {Four-photon microwave laser spectroscopy of aqueous solutions of biopolymers},
author = {Bunkin, A F and Nurmatov, A A and Pershin, S M and Khusainova, R S and Potekhin, S A},
abstractNote = {The four-photon laser radiation scattering spectra are obtained in the submillimetre range (75-95 cm{sup -1}) for deionised water, aqueous solutions of DNA and {alpha}-chymotrypsin protein. Narrow resonances are recorded whose frequencies coincide (within the resolution power of a spectrometer) with rotational frequencies in the ground electronic state and vibrational state of ortho and para isomers of H{sub 2}O molecule in a gas phase and with the frequencies of the lines of H{sub 2}O{sub 2} and OH{sup -} molecules. It is shown that the resonance contribution of the rotational lines of ortho isomers of H{sub 2}O to the signal of four-photon scattering of native solutions of biopolymers increases by a factor of at least 8 compared to their contribution to the scattering signal in water, and becomes considerably larger than the contribution from the paraisomer lines. Denaturation of DNA after heating and cooling of the solution leads to the disappearance of such selectivity. (special issue devoted to the 25th anniversary of the a.m. prokhorov general physics institute)},
doi = {10.1070/QE2007V037N10ABEH013609},
url = {https://www.osti.gov/biblio/21466736}, journal = {Quantum Electronics (Woodbury, N.Y.)},
issn = {1063-7818},
number = 10,
volume = 37,
place = {United States},
year = {Wed Oct 31 00:00:00 EDT 2007},
month = {Wed Oct 31 00:00:00 EDT 2007}
}