The inhibitory effect of superparamagnetic iron oxide nanoparticle (Ferucarbotran) on osteogenic differentiation and its signaling mechanism in human mesenchymal stem cells

Journal Article · Tue Jun 01 00:00:00 EDT 2010 · Toxicology and Applied Pharmacology

DOI:https://doi.org/10.1016/j.taap.2010.03.011· OSTI ID:21460174

Chen, Ying-Chun ^[1]; Hsiao, Jong-Kai; Liu, Hon-Man ^[2]; Lai, I-Yin ^[1]; Yao, Ming ^[3]; Hsu, Szu-Chun ^[4]; Ko, Bor-Sheng ^[3]; Chen, Yao-Chang ^[4]; Yang, Chung-Shi ^[1]; Huang, Dong-Ming ^[1]

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1. Center for Nanomedicine Research, National Health Research Institutes, Zhunan, Miaoli County 350, Taiwan (China)
2. Department of Medical Imaging, National Taiwan University Hospital and College of Medicine, National Taiwan University, Taipei 106, Taiwan (China)
3. Department of Internal Medicine, National Taiwan University Hospital and College of Medicine, National Taiwan University, Taipei 106, Taiwan (China)
4. Department of Laboratory Medicine, National Taiwan University Hospital and College of Medicine, National Taiwan University, Taipei 106, Taiwan (China)

Superparamagnetic iron oxide (SPIO) nanoparticles are very useful for monitoring cell trafficking in vivo and distinguish whether cellular regeneration originated from an exogenous cell source, which is a key issue for developing successful stem cell therapies. However, the impact of SPIO labeling on stem cell behavior remains uncertain. Here, we show the inhibitory effect of Ferucarbotran, an ionic SPIO, on osteogenic differentiation and its signaling mechanism in human mesenchymal stem cells. Ferucarbotran caused a dose-dependent inhibition of osteogenic differentiation, abolished the differentiation at high concentration, promoted cell migration, and activated the signaling molecules, {beta}-catenin, a cancer/testis antigen, SSX, and matrix metalloproteinase 2 (MMP2). An iron chelator, desferrioxamine, suppressed all the above Ferucarbotran-induced actions, demonstrating an important role of free iron in the inhibition of osteogenic differentiation that is mediated by the promotion of cell mobilization, involving the activation of a specific signaling pathway.

• OSTI ID: 21460174
• Journal Information: Toxicology and Applied Pharmacology, Vol. 245, Issue 2; Other Information: DOI: 10.1016/j.taap.2010.03.011; PII: S0041-008X(10)00097-9; Copyright (c) 2010 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; ISSN 0041-008X
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
INHIBITION
IRON OXIDES
NANOSTRUCTURES
NMR IMAGING
PARTICLES
STEM CELLS
SUPERPARAMAGNETISM
THERAPY
ANIMAL CELLS
CHALCOGENIDES
DIAGNOSTIC TECHNIQUES
IRON COMPOUNDS
MAGNETISM
MEDICINE
OXIDES
OXYGEN COMPOUNDS
SOMATIC CELLS
TRANSITION ELEMENT COMPOUNDS