Evaluation of the serum catalase and myeloperoxidase activities in chronic arsenic-exposed individuals and concomitant cytogenetic damage

Journal Article · Mon Nov 15 00:00:00 EST 2010 · Toxicology and Applied Pharmacology

DOI:https://doi.org/10.1016/j.taap.2010.08.013· OSTI ID:21451196

Banerjee, Mayukh; Banerjee, Nilanjana; Ghosh, Pritha ^[1]; Das, Jayanta K ^[2]; Basu, Santanu ^[3]; Sarkar, Ajoy K ^[4]

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1. Molecular and Human Genetics Division, Indian Institute of Chemical Biology, Kolkata (India)
2. Department of Dermatology, West Bank Hospital, Howrah (India)
3. Department of General Medicine, Sri Aurobindo Seva Kendra, Kolkata (India)
4. Peerless Hospital and B.K Roy Research Centre, Kolkata (India)

Chronic arsenic exposure through contaminated drinking water is a major environmental health issue. Chronic arsenic exposure is known to exert its toxic effects by a variety of mechanisms, of which generation of reactive oxygen species (ROS) is one of the most important. A high level of ROS, in turn, leads to DNA damage that might ultimately culminate in cancer. In order to keep the level of ROS in balance, an array of enzymes is present, of which catalase (CAT) and myeloperoxidase (MPO) are important members. Hence, in this study, we determined the activities of these two enzymes in the sera and chromosomal aberrations (CA) in peripheral blood lymphocytes in individuals exposed and unexposed to arsenic in drinking water. Arsenic in drinking water and in urine was used as a measure of exposure. Our results show that individuals chronically exposed to arsenic have significantly higher CAT and MPO activities and higher incidence of CA. We found moderate positive correlations between CAT and MPO activities, induction of CA and arsenic in urine and water. These results indicate that chronic arsenic exposure causes higher CAT and MPO activities in serum that correlates with induction of genetic damage. We conclude that the serum levels of these enzymes might be used as biomarkers of early arsenic exposure induced disease much before the classical dermatological symptoms of arsenicosis begin to appear.

• OSTI ID: 21451196
• Journal Information: Toxicology and Applied Pharmacology, Vol. 249, Issue 1; Other Information: DOI: 10.1016/j.taap.2010.08.013; PII: S0041-008X(10)00298-X; Copyright (c) 2010 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; ISSN 0041-008X
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS
ARSENIC
CATALASE
CHROMOSOMAL ABERRATIONS
CHRONIC EXPOSURE
DAMAGE
DNA DAMAGES
DRINKING WATER
LYMPHOCYTES
TOXICITY
ANIMAL CELLS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CONNECTIVE TISSUE CELLS
ELEMENTS
ENZYMES
HYDROGEN COMPOUNDS
LEUKOCYTES
MATERIALS
MUTATIONS
ORGANIC COMPOUNDS
OXIDOREDUCTASES
OXYGEN COMPOUNDS
PEROXIDASES
PROTEINS
SEMIMETALS
SOMATIC CELLS
WATER