Usefulness of MRI to Differentiate Between Temporary and Long-Term Coronary Artery Occlusion in a Minimally Invasive Model of Experimental Myocardial Infarction

Journal Article · Tue Sep 15 00:00:00 EDT 2009 · Cardiovascular and Interventional Radiology

DOI:https://doi.org/10.1007/S00270-009-9596-5· OSTI ID:21429107

Abegunewardene, Nico; Vosseler, Markus; Gori, Tommaso ^[1]; Hoffmann, Nico ^[2]; Schmidt, Kai-Helge; Becker, Dietmar ^[1]; Kreitner, Karl-Friedrich ^[3]; Petersen, Steffen E ^[4]; Schreiber, Laura M ^[2]; Horstick, Georg; Muenzel, Thomas ^[1]

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1. Johannes Gutenberg-University Mainz, Second Medical Clinic (Germany)
2. Johannes Gutenberg-University Mainz, Section of Medical Physics (Germany)
3. Johannes Gutenberg-University Mainz, Clinic for Radiology (Germany)
4. John Radcliffe Hospital, University of Oxford Centre for Clinical Magnetic Resonance Research (OCMR) (United Kingdom)

The surgical technique employed to determine an experimental ischemic damage is a major factor in the subsequent process of myocardial scar development. We set out to establish a minimally invasive porcine model of myocardial infarction using cardiac contrast-enhanced magnetic resonance imaging (ce-MRI) as the basic diagnostic tool. Twenty-seven domestic pigs were randomized to either temporary or permanent occlusion of the left anterior descending artery (LAD). Temporary occlusion was achieved by inflation of a percutaneous balloon in the left anterior descending artery directly beyond the second diagonal branch. Occlusion was maintained for 30 or 45 min, followed by reperfusion. Permanent occlusion was achieved via thrombin injection. Thirteen animals died peri- or postinterventionally due to arrhythmias. Fourteen animals survived the 30-min ischemia (four animals; group 1), the 45-min ischemia (six animals; group 2), or the permanent occlusion (4 animals; group 3). Coronary angiography and ce-MRI were performed 8 weeks after coronary occlusion to document the coronary flow grade and the size of myocardial scar tissue. The LAD was patent in all animals in groups 1 and 2, with normal TIMI flow; in group 3 animals, the LAD was totally occluded. Fibrosis of the left ventricle in group 1 (4.9 {+-} 4.4%; p = 0.008) and group 2 (9.4 {+-} 2.9%; p = 0.05) was significantly lower than in group 3 (14.5 {+-} 3.9%). Wall thickness of the ischemic area was significantly lower in group 3 versus group 1 and group 2 (2.9 {+-} 0.3, 5.9 {+-} 0.7, and 6.1 {+-} 0.7 mm; p = 0.005). The extent of late enhancement of the left ventricle was also significantly higher in group 3 (16.9 {+-} 2.1%) compared to group 1 (5.3 {+-} 5.4%; p = 0.003) and group 2 (9.7 {+-} 3.4%, p = 0.013). In conclusion, the present model of minimally invasive infarction coupled with ce-MRI may represent a useful alternative to the open chest model for studies of myocardial infarction and scar development.

• OSTI ID: 21429107
• Journal Information: Cardiovascular and Interventional Radiology, Vol. 32, Issue 5; Other Information: DOI: 10.1007/s00270-009-9596-5; Copyright (c) 2009 Springer Science+Business Media, LLC and the Cardiovascular and Interventional Radiological Society of Europe (CIRSE); ISSN 0174-1551
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
BIOMEDICAL RADIOGRAPHY
CHEST
CORONARIES
FIBROSIS
INJECTION
ISCHEMIA
MYOCARDIAL INFARCTION
NMR IMAGING
SURGERY
SWINE
THROMBIN
ANEMIAS
ANIMALS
ARTERIES
BLOOD COAGULATION FACTORS
BLOOD VESSELS
BODY
CARDIOVASCULAR DISEASES
CARDIOVASCULAR SYSTEM
DIAGNOSTIC TECHNIQUES
DISEASES
DOMESTIC ANIMALS
ENZYMES
HEMIC DISEASES
HYDROLASES
INTAKE
MAMMALS
MEDICINE
NUCLEAR MEDICINE
ORGANIC COMPOUNDS
ORGANS
PATHOLOGICAL CHANGES
PEPTIDE HYDROLASES
PROTEINS
RADIOLOGY
SERINE PROTEINASES
SYMPTOMS
VASCULAR DISEASES
VERTEBRATES