Amino Acids Profiles in Biological Media

Journal Article · Wed Aug 04 00:00:00 EDT 2010 · AIP Conference Proceedings

DOI:https://doi.org/10.1063/1.3482230· OSTI ID:21426508

Iordache, A ^[1]; Horj, E; Morar, S; Cozar, O; Culea, M ^[2]; Ani, A R ^[3]; Mesaros, C ^[4]

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1. National R and D Institute of Cryogenics and Isotopic Technologies-ICSI Rm. Valcea, 4 Uzinei St., Rm. Valcea, 240050 (Romania)
2. 'Babes-Bolyai' University, 1 M. Kogalniceanu St., Cluj-Napoca, 400084 (Romania)
3. University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca-USAMV, 3-5 Manastur St., 400372 Cluj-Napoca (Romania)
4. University of Medicine and Pharmacy, 38 Gh. Marinescu St., 540000, Targu Mures (Romania)

An accurate analytical method was developed to determine amino acids in some biological specimens by GC/MS technique. Stable isotopes provide useful tools for a variety of studies, offering ideal internal standards in quantitative information. Isotopic dilution gas chromatography--mass spectrometry (ID-GC/MS) is the techniques used for quantitative analysis of compounds labeled with stable isotopes. A Trace DSQ Thermo Finnigan quadrupole mass spectrometer coupled with a Trace GC was used. Amino acids were separated on a Rtx-5 MS capillary column, 30 mx0.25 mm, 0.25 {mu}m film thickness, using a temperature program from 50 deg. C, 1 min, 6 deg. C/min at 100 deg. C, 4 deg. C/min at 200 deg. C, 20 deg. C/min at 300 deg. C, (3 min). The transfer line temperature was 250 deg. C, the injector temperature 200 deg. C and ion source temperature 250 deg. C; splitter: 10:1. Electron energy was 70 eV and emission current, 100 {mu}A. The amino acids were purified on a Dowex 50W-W8 exchange resin and were derivatized in a procedure following two steps to obtain trifluoroacetyl butyl esters. The identification of amino acids was obtained by using NIST library but also by using amino acid standards.

• OSTI ID: 21426508
• Journal Information: AIP Conference Proceedings, Vol. 1262, Issue 1; Conference: TIM-09: Physics conference, Timisoara (Romania), 27-28 Nov 2010; Other Information: DOI: 10.1063/1.3482230; (c) 2010 American Institute of Physics; ISSN 0094-243X
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC
ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
60 APPLIED LIFE SCIENCES
AMINO ACIDS
BIOLOGICAL MATERIALS
DERIVATIZATION
ESTERS
GAS CHROMATOGRAPHY
ISOTOPE DILUTION
LABELLED COMPOUNDS
MASS SPECTROSCOPY
ORGANIC ION EXCHANGERS
PROTEINS
QUANTITATIVE CHEMICAL ANALYSIS
RESINS
STABLE ISOTOPES
CARBOXYLIC ACIDS
CHEMICAL ANALYSIS
CHEMICAL REACTIONS
CHROMATOGRAPHY
ION EXCHANGE MATERIALS
ISOTOPE APPLICATIONS
ISOTOPES
MATERIALS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC POLYMERS
PETROCHEMICALS
PETROLEUM PRODUCTS
POLYMERS
SEPARATION PROCESSES
SPECTROSCOPY
TRACER TECHNIQUES