skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Bystander Effects Induced by Medium From Irradiated Cells: Similar Transcriptome Responses in Irradiated and Bystander K562 Cells

Abstract

Purpose: Cells exposed to ionizing radiation release factors that induce deoxyribonucleic acid damage, chromosomal instability, apoptosis, and changes in the proliferation rate of neighboring unexposed cells, phenomena known as bystander effects. This work analyzes and compares changes in global transcript levels induced by direct irradiation and by bystander effects in K562 (human erythroleukemia) cells. Methods and Materials: Cells were X-irradiated with 4 Gy or transferred into culture medium collected from cells 1 h after irradiation (irradiation-conditioned medium). Global transcript profiles were assessed after 36 h of growth by use of Affymetrix microarrays (Affymetrix, Santa Clara, CA) and the kinetics of change of selected transcripts by quantitative reverse transcriptase-polymerase chain reaction. Results: The level of the majority (72%) of transcripts changed similarly (increase, decrease, or no change) in cells grown in irradiation-conditioned medium or irradiated, whereas only 0.6% showed an opposite response. Transcript level changes in bystander and irradiated cells were significantly different from those in untreated cells grown for the same amount of time and were confirmed by quantitative reverse transcriptase-polymerase chain reaction for selected genes. Signaling pathways in which the highest number of transcripts changed in both conditions were found in the following groups: neuroactive ligand-receptor, cytokine-cytokine receptor interaction,more » Janus Kinase-Signal Transducers and Activators of Transcription (JAK-STAT) and Mitogen-Activated Protein Kinase (MAPK) In control cells more transcripts were downregulated than in irradiated and bystander cells with transcription factors YBX1 and STAT5B, heat shock protein HSPA1A, and ribonucleic acid helicase DDX3X as examples. Conclusions: The transcriptomes of cells grown in medium from X-irradiated cells or directly irradiated show very similar changes. Signals released by irradiated cells may cause changes in the transcriptome of neighboring cells that sustain their survival.« less

Authors:
;  [1]; ;  [2];  [1];  [2];  [3];  [1]
  1. Department of Experimental and Clinical Radiobiology, Maria Sklodowska-Curie Memorial Cancer Center and Institute of Oncology, Gliwice (Poland)
  2. System Engineering Group, Institute of Automatic Control, Silesian University of Technology, Gliwice (Poland)
  3. Laval University Cancer Research Centre, Hotel-Dieu Hospital, Quebec, QC (Canada)
Publication Date:
OSTI Identifier:
21372266
Resource Type:
Journal Article
Journal Name:
International Journal of Radiation Oncology, Biology and Physics
Additional Journal Information:
Journal Volume: 77; Journal Issue: 1; Other Information: DOI: 10.1016/j.ijrobp.2009.11.033; PII: S0360-3016(09)03574-3; Copyright (c) 2010 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Journal ID: ISSN 0360-3016
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS; APOPTOSIS; BIOLOGICAL RADIATION EFFECTS; HEAT-SHOCK PROTEINS; IONIZING RADIATIONS; IRRADIATION; POLYMERASE CHAIN REACTION; TRANSCRIPTION FACTORS; BIOLOGICAL EFFECTS; GENE AMPLIFICATION; ORGANIC COMPOUNDS; PROTEINS; RADIATION EFFECTS; RADIATIONS

Citation Formats

Herok, Robert, Konopacka, Maria, Polanska, Joanna, Swierniak, Andrzej, Rogolinski, Jacek, Jaksik, Roman, Hancock, Ronald, Rzeszowska-Wolny, Joanna, and System Engineering Group, Institute of Automatic Control, Silesian University of Technology, Gliwice. Bystander Effects Induced by Medium From Irradiated Cells: Similar Transcriptome Responses in Irradiated and Bystander K562 Cells. United States: N. p., 2010. Web. doi:10.1016/j.ijrobp.2009.11.033.
Herok, Robert, Konopacka, Maria, Polanska, Joanna, Swierniak, Andrzej, Rogolinski, Jacek, Jaksik, Roman, Hancock, Ronald, Rzeszowska-Wolny, Joanna, & System Engineering Group, Institute of Automatic Control, Silesian University of Technology, Gliwice. Bystander Effects Induced by Medium From Irradiated Cells: Similar Transcriptome Responses in Irradiated and Bystander K562 Cells. United States. https://doi.org/10.1016/j.ijrobp.2009.11.033
Herok, Robert, Konopacka, Maria, Polanska, Joanna, Swierniak, Andrzej, Rogolinski, Jacek, Jaksik, Roman, Hancock, Ronald, Rzeszowska-Wolny, Joanna, and System Engineering Group, Institute of Automatic Control, Silesian University of Technology, Gliwice. 2010. "Bystander Effects Induced by Medium From Irradiated Cells: Similar Transcriptome Responses in Irradiated and Bystander K562 Cells". United States. https://doi.org/10.1016/j.ijrobp.2009.11.033.
@article{osti_21372266,
title = {Bystander Effects Induced by Medium From Irradiated Cells: Similar Transcriptome Responses in Irradiated and Bystander K562 Cells},
author = {Herok, Robert and Konopacka, Maria and Polanska, Joanna and Swierniak, Andrzej and Rogolinski, Jacek and Jaksik, Roman and Hancock, Ronald and Rzeszowska-Wolny, Joanna and System Engineering Group, Institute of Automatic Control, Silesian University of Technology, Gliwice},
abstractNote = {Purpose: Cells exposed to ionizing radiation release factors that induce deoxyribonucleic acid damage, chromosomal instability, apoptosis, and changes in the proliferation rate of neighboring unexposed cells, phenomena known as bystander effects. This work analyzes and compares changes in global transcript levels induced by direct irradiation and by bystander effects in K562 (human erythroleukemia) cells. Methods and Materials: Cells were X-irradiated with 4 Gy or transferred into culture medium collected from cells 1 h after irradiation (irradiation-conditioned medium). Global transcript profiles were assessed after 36 h of growth by use of Affymetrix microarrays (Affymetrix, Santa Clara, CA) and the kinetics of change of selected transcripts by quantitative reverse transcriptase-polymerase chain reaction. Results: The level of the majority (72%) of transcripts changed similarly (increase, decrease, or no change) in cells grown in irradiation-conditioned medium or irradiated, whereas only 0.6% showed an opposite response. Transcript level changes in bystander and irradiated cells were significantly different from those in untreated cells grown for the same amount of time and were confirmed by quantitative reverse transcriptase-polymerase chain reaction for selected genes. Signaling pathways in which the highest number of transcripts changed in both conditions were found in the following groups: neuroactive ligand-receptor, cytokine-cytokine receptor interaction, Janus Kinase-Signal Transducers and Activators of Transcription (JAK-STAT) and Mitogen-Activated Protein Kinase (MAPK) In control cells more transcripts were downregulated than in irradiated and bystander cells with transcription factors YBX1 and STAT5B, heat shock protein HSPA1A, and ribonucleic acid helicase DDX3X as examples. Conclusions: The transcriptomes of cells grown in medium from X-irradiated cells or directly irradiated show very similar changes. Signals released by irradiated cells may cause changes in the transcriptome of neighboring cells that sustain their survival.},
doi = {10.1016/j.ijrobp.2009.11.033},
url = {https://www.osti.gov/biblio/21372266}, journal = {International Journal of Radiation Oncology, Biology and Physics},
issn = {0360-3016},
number = 1,
volume = 77,
place = {United States},
year = {Sat May 01 00:00:00 EDT 2010},
month = {Sat May 01 00:00:00 EDT 2010}
}