skip to main content

Title: Prostate-Specific Natural Health Products (Dietary Supplements) Radiosensitize Normal Prostate Cells

Purpose: Prostate-specific health products (dietary supplements) are taken by cancer patients to alleviate the symptoms linked with poor prostate health. However, the effect of these agents on evidence-based radiotherapy practice is poorly understood. The present study aimed to determine whether dietary supplements radiosensitized normal prostate or prostate cancer cell lines. Methods and Materials: Three well-known prostate-specific dietary supplements were purchased from commercial sources available to patients (Trinovin, Provelex, and Prostate Rx). The cells used in the study included normal prostate lines (RWPE-1 and PWR-1E), prostate tumor lines (PC3, DU145, and LNCaP), and a normal nonprostate line (HaCaT). Supplement toxicity was assessed using cell proliferation assays [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] and cellular radiosensitivity using conventional clonogenic assays (0.5-4Gy). Cell cycle kinetics were assessed using the bromodeoxyuridine/propidium iodide pulse-labeling technique, apoptosis by scoring caspase-3 activation, and DNA repair by assessing gammaH2AX. Results: The cell growth and radiosensitivity of the malignant PC3, DU145, and LNcaP cells were not affected by any of the dietary prostate supplements (Provelex [2mug/mL], Trinovin [10mug/mL], and Prostate Rx [50 mug/mL]). However, both Trinovin (10mug/mL) and Prostate Rx (6mug/mL) inhibited the growth rate of the normal prostate cell lines. Prostate Rx increased cellular radiosensitivity of RWPE-1 cells through the inhibitionmore » of DNA repair. Conclusion: The use of prostate-specific dietary supplements should be discouraged during radiotherapy owing to the preferential radiosensitization of normal prostate cells.« less
Authors:
; ; ;  [1] ;  [1]
  1. William Beaumont Hospital, Royal Oak, MI (United States)
Publication Date:
OSTI Identifier:
21372117
Resource Type:
Journal Article
Resource Relation:
Journal Name: International Journal of Radiation Oncology, Biology and Physics; Journal Volume: 76; Journal Issue: 3; Other Information: DOI: 10.1016/j.ijrobp.2009.09.048; PII: S0360-3016(09)03326-4; Copyright (c) 2010 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS; 62 RADIOLOGY AND NUCLEAR MEDICINE; APOPTOSIS; BUDR; CELL CYCLE; CELL PROLIFERATION; DNA REPAIR; NEOPLASMS; PROSTATE; RADIOSENSITIVITY; RADIOTHERAPY; TETRAZOLIUM; TOXICITY ANTIMETABOLITES; AZINES; AZOLES; BIOLOGICAL RECOVERY; BIOLOGICAL REPAIR; BODY; BROMOURACILS; CHLORIDES; CHLORINE COMPOUNDS; DISEASES; DRUGS; GLANDS; HALIDES; HALOGEN COMPOUNDS; HETEROCYCLIC COMPOUNDS; HYDROXY COMPOUNDS; MALE GENITALS; MEDICINE; NUCLEAR MEDICINE; NUCLEOSIDES; NUCLEOTIDES; ORGANIC BROMINE COMPOUNDS; ORGANIC COMPOUNDS; ORGANIC HALOGEN COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; ORGANS; PYRIMIDINES; RADIOLOGY; REPAIR; RIBOSIDES; SENSITIVITY; TETRAZOLES; THERAPY; URACILS