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Title: Dielectric barrier discharge plasma treatment on E. coli: Influence of CH{sub 4}/N{sub 2}, O{sub 2}, N{sub 2}/O{sub 2}, N{sub 2}, and Ar gases

Abstract

Atmospheric pressure dielectric barrier discharge (DBD) plasma has been employed on Gram-negative bacteria, Escherichia coli BL21. Treatment was carried out using plasma generated with different compositions of gases: CH{sub 4}/N{sub 2} (1:2), O{sub 2}, N{sub 2}/O{sub 2} (1:1), N{sub 2}, and Ar, and by varying plasma power and treatment time. E. coli cells were exposed under the DBD plasma in triplicates, and their surviving numbers were observed in terms of colony forming units. It has been observed that the CH{sub 4}/N{sub 2} plasma exhibits relatively higher sterilization property toward E. coli compared to plasma generated by using O{sub 2}, N{sub 2}/O{sub 2}, N{sub 2}, and Ar gas mixtures. The time to kill up to 90% of the initial population of the E. coli cells was found to be about 2-3 min for CH{sub 4}/N{sub 2} and O{sub 2} gas mixture DBD plasma. A prolongation of treatment time and an increase in the dissipated power significantly improved the E. coli killing efficiency of the atmospheric pressure DBD plasma.

Authors:
;  [1]; ;  [2]
  1. Institute for Physics, Ernst-Moritz-Arndt-University Greifswald, Felix-Hausdorff-Str. 6, 17489 Greifswald (Germany)
  2. Institute for Biochemistry, Ernst-Moritz-Arndt-University Greifswald, Felix-Hausdorff-Str. 4, 17489 Greifswald (Germany)
Publication Date:
OSTI Identifier:
21361895
Resource Type:
Journal Article
Journal Name:
Journal of Applied Physics
Additional Journal Information:
Journal Volume: 106; Journal Issue: 8; Other Information: DOI: 10.1063/1.3236570; (c) 2009 American Institute of Physics; Journal ID: ISSN 0021-8979
Country of Publication:
United States
Language:
English
Subject:
71 CLASSICAL AND QUANTUM MECHANICS, GENERAL PHYSICS; ARGON; ATMOSPHERIC PRESSURE; BIOPHYSICS; COLONY FORMING UNITS; DECONTAMINATION; DIELECTRIC MATERIALS; ELECTRIC DISCHARGES; ESCHERICHIA COLI; METHANE; NITROGEN; OXYGEN; PLASMA; POPULATIONS; STERILIZATION; SURGERY; ALKANES; BACTERIA; CLEANING; ELEMENTS; FLUIDS; GASES; HYDROCARBONS; MATERIALS; MEDICINE; MICROORGANISMS; NONMETALS; ORGANIC COMPOUNDS; PHYSICS; RARE GASES

Citation Formats

Majumdar, Abhijit, Hippler, Rainer, Singh, Rajesh Kumar, and Palm, Gottfried J. Dielectric barrier discharge plasma treatment on E. coli: Influence of CH{sub 4}/N{sub 2}, O{sub 2}, N{sub 2}/O{sub 2}, N{sub 2}, and Ar gases. United States: N. p., 2009. Web. doi:10.1063/1.3236570.
Majumdar, Abhijit, Hippler, Rainer, Singh, Rajesh Kumar, & Palm, Gottfried J. Dielectric barrier discharge plasma treatment on E. coli: Influence of CH{sub 4}/N{sub 2}, O{sub 2}, N{sub 2}/O{sub 2}, N{sub 2}, and Ar gases. United States. https://doi.org/10.1063/1.3236570
Majumdar, Abhijit, Hippler, Rainer, Singh, Rajesh Kumar, and Palm, Gottfried J. 2009. "Dielectric barrier discharge plasma treatment on E. coli: Influence of CH{sub 4}/N{sub 2}, O{sub 2}, N{sub 2}/O{sub 2}, N{sub 2}, and Ar gases". United States. https://doi.org/10.1063/1.3236570.
@article{osti_21361895,
title = {Dielectric barrier discharge plasma treatment on E. coli: Influence of CH{sub 4}/N{sub 2}, O{sub 2}, N{sub 2}/O{sub 2}, N{sub 2}, and Ar gases},
author = {Majumdar, Abhijit and Hippler, Rainer and Singh, Rajesh Kumar and Palm, Gottfried J},
abstractNote = {Atmospheric pressure dielectric barrier discharge (DBD) plasma has been employed on Gram-negative bacteria, Escherichia coli BL21. Treatment was carried out using plasma generated with different compositions of gases: CH{sub 4}/N{sub 2} (1:2), O{sub 2}, N{sub 2}/O{sub 2} (1:1), N{sub 2}, and Ar, and by varying plasma power and treatment time. E. coli cells were exposed under the DBD plasma in triplicates, and their surviving numbers were observed in terms of colony forming units. It has been observed that the CH{sub 4}/N{sub 2} plasma exhibits relatively higher sterilization property toward E. coli compared to plasma generated by using O{sub 2}, N{sub 2}/O{sub 2}, N{sub 2}, and Ar gas mixtures. The time to kill up to 90% of the initial population of the E. coli cells was found to be about 2-3 min for CH{sub 4}/N{sub 2} and O{sub 2} gas mixture DBD plasma. A prolongation of treatment time and an increase in the dissipated power significantly improved the E. coli killing efficiency of the atmospheric pressure DBD plasma.},
doi = {10.1063/1.3236570},
url = {https://www.osti.gov/biblio/21361895}, journal = {Journal of Applied Physics},
issn = {0021-8979},
number = 8,
volume = 106,
place = {United States},
year = {Thu Oct 15 00:00:00 EDT 2009},
month = {Thu Oct 15 00:00:00 EDT 2009}
}