A peptide that binds the pea aphid gut impedes entry of Pea enation mosaic virus into the aphid hemocoel
Abstract
Development of ways to block virus transmission by aphids could lead to novel and broad-spectrum means of controlling plant viruses. Viruses in the Luteoviridae enhanced are obligately transmitted by aphids in a persistent manner that requires virion accumulation in the aphid hemocoel. To enter the hemocoel, the virion must bind and traverse the aphid gut epithelium. By screening a phage display library, we identified a 12-residue gut binding peptide (GBP3.1) that binds to the midgut and hindgut of the pea aphid Acyrthosiphon pisum. Binding was confirmed by labeling the aphid gut with a GBP3.1-green fluorescent protein fusion. GBP3.1 reduced uptake of Pea enation mosaic virus (Luteoviridae) from the pea aphid gut into the hemocoel. GBP3.1 also bound to the gut epithelia of the green peach aphid and the soybean aphid. These results suggest a novel strategy for inhibiting plant virus transmission by at least three major aphid pest species.
- Authors:
-
- Department of Entomology, Iowa State University, Ames, IA 50011 (United States)
- Publication Date:
- OSTI Identifier:
- 21357616
- Resource Type:
- Journal Article
- Journal Name:
- Virology
- Additional Journal Information:
- Journal Volume: 401; Journal Issue: 1; Other Information: DOI: 10.1016/j.virol.2010.02.009; PII: S0042-6822(10)00109-1; Copyright (c) 2010 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Journal ID: ISSN 0042-6822
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 60 APPLIED LIFE SCIENCES; APHIDS; BACTERIOPHAGES; BUILDUP; EPITHELIUM; PEACHES; PEAS; PEPTIDES; PISUM; SOYBEANS; TRANSMISSION; ANIMAL TISSUES; ANIMALS; ARTHROPODS; BODY; FOOD; FRUITS; HEMIPTERA; INSECTS; INVERTEBRATES; LEGUMINOSAE; MAGNOLIOPHYTA; MAGNOLIOPSIDA; MICROORGANISMS; ORGANIC COMPOUNDS; PARASITES; PLANTS; PROTEINS; SEEDS; VEGETABLES; VIRUSES
Citation Formats
Liu Sijun, E-mail: sliu@iastate.ed, Sivakumar, S, Sparks, Wendy O., E-mail: wosparks@iastate.ed, Interdepartmental Graduate Genetics Program, Iowa State University, Ames, IA 50011, Miller, W. Allen, E-mail: wamiller@iastate.ed, and Bonning, Bryony C., E-mail: bbonning@iastate.ed. A peptide that binds the pea aphid gut impedes entry of Pea enation mosaic virus into the aphid hemocoel. United States: N. p., 2010.
Web.
Liu Sijun, E-mail: sliu@iastate.ed, Sivakumar, S, Sparks, Wendy O., E-mail: wosparks@iastate.ed, Interdepartmental Graduate Genetics Program, Iowa State University, Ames, IA 50011, Miller, W. Allen, E-mail: wamiller@iastate.ed, & Bonning, Bryony C., E-mail: bbonning@iastate.ed. A peptide that binds the pea aphid gut impedes entry of Pea enation mosaic virus into the aphid hemocoel. United States.
Liu Sijun, E-mail: sliu@iastate.ed, Sivakumar, S, Sparks, Wendy O., E-mail: wosparks@iastate.ed, Interdepartmental Graduate Genetics Program, Iowa State University, Ames, IA 50011, Miller, W. Allen, E-mail: wamiller@iastate.ed, and Bonning, Bryony C., E-mail: bbonning@iastate.ed. 2010.
"A peptide that binds the pea aphid gut impedes entry of Pea enation mosaic virus into the aphid hemocoel". United States.
@article{osti_21357616,
title = {A peptide that binds the pea aphid gut impedes entry of Pea enation mosaic virus into the aphid hemocoel},
author = {Liu Sijun, E-mail: sliu@iastate.ed and Sivakumar, S and Sparks, Wendy O., E-mail: wosparks@iastate.ed and Interdepartmental Graduate Genetics Program, Iowa State University, Ames, IA 50011 and Miller, W. Allen, E-mail: wamiller@iastate.ed and Bonning, Bryony C., E-mail: bbonning@iastate.ed},
abstractNote = {Development of ways to block virus transmission by aphids could lead to novel and broad-spectrum means of controlling plant viruses. Viruses in the Luteoviridae enhanced are obligately transmitted by aphids in a persistent manner that requires virion accumulation in the aphid hemocoel. To enter the hemocoel, the virion must bind and traverse the aphid gut epithelium. By screening a phage display library, we identified a 12-residue gut binding peptide (GBP3.1) that binds to the midgut and hindgut of the pea aphid Acyrthosiphon pisum. Binding was confirmed by labeling the aphid gut with a GBP3.1-green fluorescent protein fusion. GBP3.1 reduced uptake of Pea enation mosaic virus (Luteoviridae) from the pea aphid gut into the hemocoel. GBP3.1 also bound to the gut epithelia of the green peach aphid and the soybean aphid. These results suggest a novel strategy for inhibiting plant virus transmission by at least three major aphid pest species.},
doi = {},
url = {https://www.osti.gov/biblio/21357616},
journal = {Virology},
issn = {0042-6822},
number = 1,
volume = 401,
place = {United States},
year = {Tue May 25 00:00:00 EDT 2010},
month = {Tue May 25 00:00:00 EDT 2010}
}