Alternate receptor usage of neuropilin-1 and glucose transporter protein 1 by the human T cell leukemia virus type 1
- Department of Microbiology and Immunology, Indiana University School of Medicine, 635 Barnhill Drive, Rm420, Indianapolis, IN (United States)
- Department of Biology, IUPUI School of Science (United States)
- Department of Medical and Molecular Genetics, Indiana University School of Medicine, 635 Barnhill Drive, Rm420, Indianapolis, IN (United States)
Recent studies have demonstrated that neuropilin 1 (NP-1) is involved in HTLV-1 entry; however, the role NP-1 plays in this process is not understood. We demonstrated that ectopic expression of human NP-1 but not NP-2 cDNA increased susceptibility to HTLV-1. SiRNA-mediated inhibition of NP-1 expression correlated with significant reduction of HTLV-1 Env-mediated fusion. The vascular endothelial growth factor (VEGF{sub 165}) caused downmodulation of surface NP-1 and inhibited HTLV-1 infection of U87 cells. In contrast, VEGF{sub 165} partially inhibited infection of primary astrocytes and had no significant effect on infection of HeLa cells. VEGF{sub 165} and antibodies to the glucose transporter protein 1 (anti-GLUT-1) were both needed to block infection of primary astrocytes, however, only anti-GLUT-1 antibodies were sufficient to block infection of HeLa cells. HTLV-1 Env forms complexes with both NP-1 and GLUT-1 in primary human astrocytes. The alternate usage of these two cellular receptors may have important implications regarding HTLV-1 neuro-tropism.
- OSTI ID:
- 21357582
- Journal Information:
- Virology, Vol. 396, Issue 2; Other Information: DOI: 10.1016/j.virol.2009.10.015; PII: S0042-6822(09)00624-2; Copyright (c) 2009 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; ISSN 0042-6822
- Country of Publication:
- United States
- Language:
- English
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ANTIBODIES
GLUCOSE
GLYCOPROTEINS
GROWTH FACTORS
HELA CELLS
INHIBITION
LEUKEMIA VIRUSES
RECEPTORS
ALDEHYDES
ANIMAL CELLS
CARBOHYDRATES
HEXOSES
MEMBRANE PROTEINS
MICROORGANISMS
MITOGENS
MONOSACCHARIDES
ONCOGENIC VIRUSES
ORGANIC COMPOUNDS
PARASITES
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SACCHARIDES
TUMOR CELLS
VIRUSES