The epithelial-mesenchymal transition induced by keratinocyte growth conditions is overcome by E6 and E7 from HPV16, but not HPV8 and HPV38: Characterization of global transcription profiles

Journal Article · Fri Jun 05 00:00:00 EDT 2009 · Virology

DOI:https://doi.org/10.1016/j.virol.2009.03.033· OSTI ID:21357520

Azzimonti, Barbara; Dell'Oste, Valentina; Borgogna, Cinzia; Mondini, Michele ^[1]; Gugliesi, Francesca ^[2]; De Andrea, Marco ^[1]; Chiorino, Giovanna; Scatolini, Maria; Ghimenti, Chiara ^[3]; Landolfo, Santo ^[2]; Gariglio, Marisa ^[1]

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1. Department of Clinical and Experimental Medicine, Medical School of Novara, via Solaroli 17, 28100 Novara (Italy)
2. Department of Public Health and Microbiology, Medical School of Turin, via Santena 9, 10126 Torino (Italy)
3. Cancer Genomics Lab Fondo Edo Tempia, via Malta 3, 13900 Biella (Italy)

The aim of this study was to evaluate the growth properties of primary human keratinocytes expressing E6 and E7 proteins, which are from either the beta- or alpha-genotypes, under different culture conditions. We demonstrated that keratinocytes expressing E6 and E7, from both HPV8 and 38, irreversibly underwent the epithelial-mesenchymal transition (EMT) when grown on plastic with FAD medium (F12/DMEM/5%FBS). Expression of E6/E7 from HPV16 was capable of fully overcoming the FAD-induced EMT. Immortalization was only observed in HPV16-transduced cell lines, while the more proliferating phenotype of both KerHPV8 and 38 was mainly related to FAD-induced EMT. Microarray analysis of exponentially growing cells identified 146 cellular genes that were differentially regulated in HPV16 compared to HPV8- and 38-transduced cells. A large accumulation of transcripts associated with epidermal development and differentiation was observed in HPV16-transduced cells, whereas transcripts of genes involved in the extracellular matrix, multicellular organismal processes, and inflammatory response were affected in HPV8 and 38-transduced cells.

• OSTI ID: 21357520
• Journal Information: Virology, Vol. 388, Issue 2; Other Information: DOI: 10.1016/j.virol.2009.03.033; PII: S0042-6822(09)00232-3; Copyright (c) 2009 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; ISSN 0042-6822
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
BUILDUP
EPITHELIUM
GENES
GENOTYPE
GROWTH
INFLAMMATION
PROTEINS
TRANSCRIPTION
VIRUSES
ANIMAL TISSUES
BODY
MICROORGANISMS
ORGANIC COMPOUNDS
PARASITES
PATHOLOGICAL CHANGES
SYMPTOMS