Chromium picolinate induced apoptosis of lymphocytes and the signaling mechanisms thereof
- Biochemistry Laboratory, Central Leather Research Institute, Adyar, Chennai 600 020 (India)
- Biophysics Laboratory, Central Leather Research Institute, Adyar, Chennai (India)
Cr(III)(picolinate){sub 3} [Cr(III)(pic){sub 3}] is currently used as a nutritional supplement and for treating Type-2 diabetes. The effect of Cr(III)(pic){sub 3} uptake in peripheral blood lymphocytes is investigated in this study. From the cytotoxicity data, DNA fragmentation pattern, Annexin V staining, TUNEL positivity and the ultrastructural characteristics such as chromatin condensation and formation of apoptotic bodies, it is clear that Cr(III)(pic){sub 3} induces a concentration dependent apoptosis. It is shown that reactive oxygen species (ROS) produced by treatment with Cr(III)(pic){sub 3} leads to apoptosis, since we find that pretreatment with N-acetyl cysteine inhibits the process. Using Western blotting technique and fluorescence measurements, the downstream signaling molecules have also been identified. Cr(III)(pic){sub 3} treatment leads to collapse of the mitochondrial membrane potential, Bax expression, increase in cytosolic cytochrome c content and active caspase-3 and DNA fragmentation and all these manifestations are reduced by pretreating the lymphocytes with N-acetyl cysteine. Thus, it is shown that Cr(III)(pic){sub 3} is cytotoxic to lymphocytes with ROS and mitochondrial events playing a role in bringing about apoptosis.
- OSTI ID:
- 21272582
- Journal Information:
- Toxicology and Applied Pharmacology, Vol. 237, Issue 3; Other Information: DOI: 10.1016/j.taap.2009.04.006; PII: S0041-008X(09)00149-5; Copyright (c) 2009 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0041-008X
- Country of Publication:
- United States
- Language:
- English
Similar Records
Low concentration of arsenic could induce caspase-3 mediated head kidney macrophage apoptosis with JNK-p38 activation in Clarias batrachus
Cannabidiol-induced apoptosis in primary lymphocytes is associated with oxidative stress-dependent activation of caspase-8