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Title: Utilization of molasses spentwash for production of bioplastics by waste activated sludge

Abstract

Present study describes the treatment of molasses spentwash and its use as a potential low cost substrate for production of biopolymer polyhydroxybutyrate (PHB) by waste activated sludge. Fluorescence microscopy revealed the presence of PHB granules in sludge biomass which was further confirmed by fourier transform-infra-red spectroscopy (FT-IR) and {sup 13}C nuclear magnetic resonance (NMR). The processing of molasses spentwash was carried out for attaining different ratios of carbon and nitrogen (C:N). Highest chemical oxygen demand (COD) removal and PHB accumulation of 60% and 31% respectively was achieved with raw molasses spentwash containing inorganic nitrogen (C:N ratio = 28) followed by COD removal of 52% and PHB accumulation of 28% for filtered molasses containing inorganic nitrogen (C:N ratio = 29). PHB production yield (Y{sub p/s}) was highest (0.184 g g{sup -1} COD consumed) for deproteinized spentwash supplemented with nitrogen. In contrast, the substrate consumption and product formation were higher in case of raw spentwash. Though COD removal was lowest from deproteinized spentwash, evaluation of kinetic parameters suggested higher rates of conversion of available carbon to biomass and PHB. Thus the process provided dual benefit of conversion of two wastes viz. waste activated sludge and molasses spentwash into value-added product-PHB.

Authors:
 [1];  [2];  [3]
  1. Solid Waste Management Unit, National Environmental Engineering Research Institute (NEERI), Nehru Marg, Nagpur 440 020 (India)
  2. Environmental Biotechnology Division, National Environmental Engineering Research Institute (NEERI), Nehru Marg, Nagpur 440 020 (India)
  3. National Environmental Engineering Research Institute (NEERI), Nehru Marg, Nagpur 440 020 (India)
Publication Date:
OSTI Identifier:
21269367
Resource Type:
Journal Article
Journal Name:
Waste Management
Additional Journal Information:
Journal Volume: 29; Journal Issue: 9; Other Information: DOI: 10.1016/j.wasman.2009.04.008; PII: S0956-053X(09)00167-6; Copyright (c) 2009 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0956-053X
Country of Publication:
United States
Language:
English
Subject:
12 MANAGEMENT OF RADIOACTIVE WASTES, AND NON-RADIOACTIVE WASTES FROM NUCLEAR FACILITIES; BIOMASS; CARBON; CARBON 13; CHEMICAL OXYGEN DEMAND; FLUORESCENCE; INFRARED SPECTRA; MICROSCOPY; MOLASSES; NITROGEN; NUCLEAR MAGNETIC RESONANCE; SPECTROSCOPY; WASTES

Citation Formats

Khardenavis, Anshuman A., Vaidya, Atul N, Kumar, M Suresh, and Chakrabarti, Tapan. Utilization of molasses spentwash for production of bioplastics by waste activated sludge. United States: N. p., 2009. Web. doi:10.1016/j.wasman.2009.04.008.
Khardenavis, Anshuman A., Vaidya, Atul N, Kumar, M Suresh, & Chakrabarti, Tapan. Utilization of molasses spentwash for production of bioplastics by waste activated sludge. United States. https://doi.org/10.1016/j.wasman.2009.04.008
Khardenavis, Anshuman A., Vaidya, Atul N, Kumar, M Suresh, and Chakrabarti, Tapan. 2009. "Utilization of molasses spentwash for production of bioplastics by waste activated sludge". United States. https://doi.org/10.1016/j.wasman.2009.04.008.
@article{osti_21269367,
title = {Utilization of molasses spentwash for production of bioplastics by waste activated sludge},
author = {Khardenavis, Anshuman A. and Vaidya, Atul N and Kumar, M Suresh and Chakrabarti, Tapan},
abstractNote = {Present study describes the treatment of molasses spentwash and its use as a potential low cost substrate for production of biopolymer polyhydroxybutyrate (PHB) by waste activated sludge. Fluorescence microscopy revealed the presence of PHB granules in sludge biomass which was further confirmed by fourier transform-infra-red spectroscopy (FT-IR) and {sup 13}C nuclear magnetic resonance (NMR). The processing of molasses spentwash was carried out for attaining different ratios of carbon and nitrogen (C:N). Highest chemical oxygen demand (COD) removal and PHB accumulation of 60% and 31% respectively was achieved with raw molasses spentwash containing inorganic nitrogen (C:N ratio = 28) followed by COD removal of 52% and PHB accumulation of 28% for filtered molasses containing inorganic nitrogen (C:N ratio = 29). PHB production yield (Y{sub p/s}) was highest (0.184 g g{sup -1} COD consumed) for deproteinized spentwash supplemented with nitrogen. In contrast, the substrate consumption and product formation were higher in case of raw spentwash. Though COD removal was lowest from deproteinized spentwash, evaluation of kinetic parameters suggested higher rates of conversion of available carbon to biomass and PHB. Thus the process provided dual benefit of conversion of two wastes viz. waste activated sludge and molasses spentwash into value-added product-PHB.},
doi = {10.1016/j.wasman.2009.04.008},
url = {https://www.osti.gov/biblio/21269367}, journal = {Waste Management},
issn = {0956-053X},
number = 9,
volume = 29,
place = {United States},
year = {Tue Sep 15 00:00:00 EDT 2009},
month = {Tue Sep 15 00:00:00 EDT 2009}
}