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Title: Phenylephrine protects autotransplanted rabbit submandibular gland from apoptosis

Abstract

Submandibular gland (SMG) autotransplantation is an effective treatment for severe keratoconjunctivitis sicca. Our previous studies have shown that phenylephrine attenuates structural injury and promotes cell proliferation in autotransplanted rabbit SMG. However, the mechanism by which phenylephrine reduces the injury has not been fully evaluated. In this study, we investigate the ability of phenylephrine to inhibit apoptosis in autotransplanted rabbit SMG. We observed that apoptosis occurred in the early phase of SMG transplantation and that phenylephrine treatment protected transplanted SMG from apoptosis. Furthermore, we found that phenylephrine could significantly upregulate the expression of Bcl-2, downregulate the expression of Bax, and inhibit the activation of both caspase-3 and p38 mitogen-activated protein kinase in autotransplanted SMG. Therefore, the cytoprotective effects of phenylephrine on autotransplanted SMG may be a novel clinical strategy for autotransplanted SMG protection during the early postoperative stage of transplantation.

Authors:
 [1]; ;  [2];  [3];  [4]
  1. Department of Oral and Maxillofacial Surgery, Peking University School and Hospital of Stomatology, 22 Zhong Guan Cun South Street, Beijing 100081 (China)
  2. Department of Physiology and Pathophysiology, Peking University Health Science Center and Key Laboratory of Molecular Cardiovascular Sciences, Ministry of Education, 38 Xue Yuan Road, Beijing 100191 (China)
  3. Department of Oral Pathology, Peking University School and Hospital of Stomatology, Beijing 100081 (China)
  4. Central Research Laboratory, Peking University School and Hospital of Stomatology, Beijing 100081 (China)
Publication Date:
OSTI Identifier:
21217127
Resource Type:
Journal Article
Journal Name:
Biochemical and Biophysical Research Communications
Additional Journal Information:
Journal Volume: 377; Journal Issue: 1; Other Information: DOI: 10.1016/j.bbrc.2008.09.120; PII: S0006-291X(08)01880-9; Copyright (c) 2008 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; APOPTOSIS; CELL PROLIFERATION; GLANDS; PROTEINS; RABBITS

Citation Formats

Bin, Xiang, Department of Oral Medicine, Medical College, Dalian University, Dalian 116622, Liaoning, Yan, Zhang, Yuming, Li, Yan, Gao, Yehua, Gan, Wu Liling, and Yu Guangyan. Phenylephrine protects autotransplanted rabbit submandibular gland from apoptosis. United States: N. p., 2008. Web. doi:10.1016/j.bbrc.2008.09.120.
Bin, Xiang, Department of Oral Medicine, Medical College, Dalian University, Dalian 116622, Liaoning, Yan, Zhang, Yuming, Li, Yan, Gao, Yehua, Gan, Wu Liling, & Yu Guangyan. Phenylephrine protects autotransplanted rabbit submandibular gland from apoptosis. United States. https://doi.org/10.1016/j.bbrc.2008.09.120
Bin, Xiang, Department of Oral Medicine, Medical College, Dalian University, Dalian 116622, Liaoning, Yan, Zhang, Yuming, Li, Yan, Gao, Yehua, Gan, Wu Liling, and Yu Guangyan. 2008. "Phenylephrine protects autotransplanted rabbit submandibular gland from apoptosis". United States. https://doi.org/10.1016/j.bbrc.2008.09.120.
@article{osti_21217127,
title = {Phenylephrine protects autotransplanted rabbit submandibular gland from apoptosis},
author = {Bin, Xiang and Department of Oral Medicine, Medical College, Dalian University, Dalian 116622, Liaoning and Yan, Zhang and Yuming, Li and Yan, Gao and Yehua, Gan and Wu Liling and Yu Guangyan},
abstractNote = {Submandibular gland (SMG) autotransplantation is an effective treatment for severe keratoconjunctivitis sicca. Our previous studies have shown that phenylephrine attenuates structural injury and promotes cell proliferation in autotransplanted rabbit SMG. However, the mechanism by which phenylephrine reduces the injury has not been fully evaluated. In this study, we investigate the ability of phenylephrine to inhibit apoptosis in autotransplanted rabbit SMG. We observed that apoptosis occurred in the early phase of SMG transplantation and that phenylephrine treatment protected transplanted SMG from apoptosis. Furthermore, we found that phenylephrine could significantly upregulate the expression of Bcl-2, downregulate the expression of Bax, and inhibit the activation of both caspase-3 and p38 mitogen-activated protein kinase in autotransplanted SMG. Therefore, the cytoprotective effects of phenylephrine on autotransplanted SMG may be a novel clinical strategy for autotransplanted SMG protection during the early postoperative stage of transplantation.},
doi = {10.1016/j.bbrc.2008.09.120},
url = {https://www.osti.gov/biblio/21217127}, journal = {Biochemical and Biophysical Research Communications},
issn = {0006-291X},
number = 1,
volume = 377,
place = {United States},
year = {Fri Dec 05 00:00:00 EST 2008},
month = {Fri Dec 05 00:00:00 EST 2008}
}