A population of human brain cells expressing phenotypic markers of more than one lineage can be induced in vitro to differentiate into mesenchymal cells
Abstract
Proliferating astrocytic cells from germinal, as well as mature areas of brain parenchyma, have the characteristics of neural stem/progenitor cells and are capable of generating both neurons and glia. We previously reported that primary fetal human brain cells, designated as Normal Human Astrocytes (NHA), expressed, in addition to GFAP, Vimentin and Nestin, low levels of {beta}III-Tubulin, an early neuronal marker, and differentiated into neurons and astrocytes in vitro. Here, we showed that primary NHA cells co-express low levels of mesenchymal markers Fibronectin and Collagen-1 in culture. These cells transitioned into mesenchymal-like cells when cultured in adherent conditions in serum containing media. The mesenchymal-like derivatives of these cells were characterized based on their morphological changes, high expression of Vimentin and extracellular matrix (ECM) proteins, Collagen-1 and Fibronectin, and decline of neural markers. When incubated in osteogenic and adipogenic induction media, the mesenchymal-like cells differentiated into osteoblasts and adipocytes. Furthermore, NHA cells express markers of neural crest cells, SOX-10 and p75. These data support the idea of ectoderm-derived mesenchymal lineages. These findings suggest that a population of primitive fetal brain cells with neural/neural crest/mesenchymal phenotype, resembles the remarkable phenotypic plasticity of neural crest cells, and differentiates into adipocytes and osteocytes under themore »
- Authors:
-
- Department of Neurology, Temple University School of Medicine, 3401 N. Broad Street, Philadelphia, Pennsylvania 19140 (United States)
- Department of Molecular Pathology and Neuropathology, Medical University Lodz (Poland)
- Department of Physiology, Temple University School of Medicine, Philadelphia, Pennsylvania 19140 (United States)
- Publication Date:
- OSTI Identifier:
- 21176171
- Resource Type:
- Journal Article
- Journal Name:
- Experimental Cell Research
- Additional Journal Information:
- Journal Volume: 315; Journal Issue: 3; Other Information: DOI: 10.1016/j.yexcr.2008.11.004; PII: S0014-4827(08)00471-0; Copyright (c) 2008 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0014-4827
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 60 APPLIED LIFE SCIENCES; BONE CELLS; BRAIN; COLLAGEN; GENES; IN VITRO; MORPHOLOGICAL CHANGES; NERVE CELLS; PHENOTYPE; PLASTICITY
Citation Formats
Rieske, Piotr, Department of Molecular Pathology and Neuropathology, Medical University Lodz, Augelli, Brian J, Stawski, Robert, Gaughan, John, Azizi, S Ausim, and Krynska, Barbara. A population of human brain cells expressing phenotypic markers of more than one lineage can be induced in vitro to differentiate into mesenchymal cells. United States: N. p., 2009.
Web. doi:10.1016/j.yexcr.2008.11.004.
Rieske, Piotr, Department of Molecular Pathology and Neuropathology, Medical University Lodz, Augelli, Brian J, Stawski, Robert, Gaughan, John, Azizi, S Ausim, & Krynska, Barbara. A population of human brain cells expressing phenotypic markers of more than one lineage can be induced in vitro to differentiate into mesenchymal cells. United States. https://doi.org/10.1016/j.yexcr.2008.11.004
Rieske, Piotr, Department of Molecular Pathology and Neuropathology, Medical University Lodz, Augelli, Brian J, Stawski, Robert, Gaughan, John, Azizi, S Ausim, and Krynska, Barbara. 2009.
"A population of human brain cells expressing phenotypic markers of more than one lineage can be induced in vitro to differentiate into mesenchymal cells". United States. https://doi.org/10.1016/j.yexcr.2008.11.004.
@article{osti_21176171,
title = {A population of human brain cells expressing phenotypic markers of more than one lineage can be induced in vitro to differentiate into mesenchymal cells},
author = {Rieske, Piotr and Department of Molecular Pathology and Neuropathology, Medical University Lodz and Augelli, Brian J and Stawski, Robert and Gaughan, John and Azizi, S Ausim and Krynska, Barbara},
abstractNote = {Proliferating astrocytic cells from germinal, as well as mature areas of brain parenchyma, have the characteristics of neural stem/progenitor cells and are capable of generating both neurons and glia. We previously reported that primary fetal human brain cells, designated as Normal Human Astrocytes (NHA), expressed, in addition to GFAP, Vimentin and Nestin, low levels of {beta}III-Tubulin, an early neuronal marker, and differentiated into neurons and astrocytes in vitro. Here, we showed that primary NHA cells co-express low levels of mesenchymal markers Fibronectin and Collagen-1 in culture. These cells transitioned into mesenchymal-like cells when cultured in adherent conditions in serum containing media. The mesenchymal-like derivatives of these cells were characterized based on their morphological changes, high expression of Vimentin and extracellular matrix (ECM) proteins, Collagen-1 and Fibronectin, and decline of neural markers. When incubated in osteogenic and adipogenic induction media, the mesenchymal-like cells differentiated into osteoblasts and adipocytes. Furthermore, NHA cells express markers of neural crest cells, SOX-10 and p75. These data support the idea of ectoderm-derived mesenchymal lineages. These findings suggest that a population of primitive fetal brain cells with neural/neural crest/mesenchymal phenotype, resembles the remarkable phenotypic plasticity of neural crest cells, and differentiates into adipocytes and osteocytes under the influence of environmental factors.},
doi = {10.1016/j.yexcr.2008.11.004},
url = {https://www.osti.gov/biblio/21176171},
journal = {Experimental Cell Research},
issn = {0014-4827},
number = 3,
volume = 315,
place = {United States},
year = {Sun Feb 01 00:00:00 EST 2009},
month = {Sun Feb 01 00:00:00 EST 2009}
}