skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: A population of serumdeprivation-induced bone marrow stem cells (SD-BMSC) expresses marker typical for embryonic and neural stem cells

Abstract

The bone marrow represents an easy accessible source of adult stem cells suitable for various cell based therapies. Several studies in recent years suggested the existence of pluripotent stem cells within bone marrow stem cells (BMSC) expressing marker proteins of both embryonic and tissue committed stem cells. These subpopulations were referred to as MAPC, MIAMI and VSEL-cells. Here we describe SD-BMSC (serumdeprivation-induced BMSC) which are induced as a distinct subpopulation after complete serumdeprivation. SD-BMSC are generated from small-sized nestin-positive BMSC (S-BMSC) organized as round-shaped cells in the top layer of BMSC-cultures. The generation of SD-BMSC is caused by a selective proliferation of S-BMSC and accompanied by changes in both morphology and gene expression. SD-BMSC up-regulate not only markers typical for neural stem cells like nestin and GFAP, but also proteins characteristic for embryonic cells like Oct4 and SOX2. We hypothesize, that SD-BMSC like MAPC, MIAMI and VSEL-cells represent derivatives from a single pluripotent stem cell fraction within BMSC exhibiting characteristics of embryonic and tissue committed stem cells. The complete removal of serum might offer a simple way to specifically enrich this fraction of pluripotent embryonic like stem cells in BMSC cultures.

Authors:
 [1]; ;  [2];  [1];  [1]
  1. Leibniz Institute for Neurobiology (IfN), Projectgroup Neuropharmacology, Brenneckestr. 6, 39118 Magdeburg (Germany)
  2. Institute for Physiology, Medizinische Fakultaet, Otto-von-Guericke-University, Leipziger Str. 44, 39120 Magdeburg (Germany)
Publication Date:
OSTI Identifier:
21176151
Resource Type:
Journal Article
Journal Name:
Experimental Cell Research
Additional Journal Information:
Journal Volume: 315; Journal Issue: 1; Other Information: DOI: 10.1016/j.yexcr.2008.10.007; PII: S0014-4827(08)00399-6; Copyright (c) 2008 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0014-4827
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; BONE MARROW; CELL PROLIFERATION; DEOXYURIDINE; EMBRYONIC CELLS; MORPHOLOGY; PH VALUE; PHOSPHATES; PROTEINS; STEM CELLS; THERAPY

Citation Formats

Sauerzweig, Steven, Research Institute for Applied Neuroscience, Munsch, Thomas, Lessmann, Volkmar, Reymann, Klaus G, Research Institute for Applied Neuroscience, and Braun, Holger. A population of serumdeprivation-induced bone marrow stem cells (SD-BMSC) expresses marker typical for embryonic and neural stem cells. United States: N. p., 2009. Web. doi:10.1016/j.yexcr.2008.10.007.
Sauerzweig, Steven, Research Institute for Applied Neuroscience, Munsch, Thomas, Lessmann, Volkmar, Reymann, Klaus G, Research Institute for Applied Neuroscience, & Braun, Holger. A population of serumdeprivation-induced bone marrow stem cells (SD-BMSC) expresses marker typical for embryonic and neural stem cells. United States. https://doi.org/10.1016/j.yexcr.2008.10.007
Sauerzweig, Steven, Research Institute for Applied Neuroscience, Munsch, Thomas, Lessmann, Volkmar, Reymann, Klaus G, Research Institute for Applied Neuroscience, and Braun, Holger. 2009. "A population of serumdeprivation-induced bone marrow stem cells (SD-BMSC) expresses marker typical for embryonic and neural stem cells". United States. https://doi.org/10.1016/j.yexcr.2008.10.007.
@article{osti_21176151,
title = {A population of serumdeprivation-induced bone marrow stem cells (SD-BMSC) expresses marker typical for embryonic and neural stem cells},
author = {Sauerzweig, Steven and Research Institute for Applied Neuroscience and Munsch, Thomas and Lessmann, Volkmar and Reymann, Klaus G and Research Institute for Applied Neuroscience and Braun, Holger},
abstractNote = {The bone marrow represents an easy accessible source of adult stem cells suitable for various cell based therapies. Several studies in recent years suggested the existence of pluripotent stem cells within bone marrow stem cells (BMSC) expressing marker proteins of both embryonic and tissue committed stem cells. These subpopulations were referred to as MAPC, MIAMI and VSEL-cells. Here we describe SD-BMSC (serumdeprivation-induced BMSC) which are induced as a distinct subpopulation after complete serumdeprivation. SD-BMSC are generated from small-sized nestin-positive BMSC (S-BMSC) organized as round-shaped cells in the top layer of BMSC-cultures. The generation of SD-BMSC is caused by a selective proliferation of S-BMSC and accompanied by changes in both morphology and gene expression. SD-BMSC up-regulate not only markers typical for neural stem cells like nestin and GFAP, but also proteins characteristic for embryonic cells like Oct4 and SOX2. We hypothesize, that SD-BMSC like MAPC, MIAMI and VSEL-cells represent derivatives from a single pluripotent stem cell fraction within BMSC exhibiting characteristics of embryonic and tissue committed stem cells. The complete removal of serum might offer a simple way to specifically enrich this fraction of pluripotent embryonic like stem cells in BMSC cultures.},
doi = {10.1016/j.yexcr.2008.10.007},
url = {https://www.osti.gov/biblio/21176151}, journal = {Experimental Cell Research},
issn = {0014-4827},
number = 1,
volume = 315,
place = {United States},
year = {Thu Jan 01 00:00:00 EST 2009},
month = {Thu Jan 01 00:00:00 EST 2009}
}