Kinetic of the Intracellular Incorporation of New Phthalocyanines Synthesized in mexico and Its Potential as Photosensibilizers in the Photodynamic Therapy
- Departamento de Morfologia, ENCB, Institute Politecnico Nacional. Carpio y Plan de Ayala S/N. Col. Sto Tomas. Mexico, D.F. C.P. 11340 (Mexico)
- Hospital Infantil de Mexico (Mexico)
- Depto. de Fisica, CINVESTAV-IPN, unidad Zacatenco, Mexico, D.F. (Mexico)
- Universidad Autonoma de Puebla. Blvd. 14 Sur y Ave. San Claudio, Edificio 137 Ciudad Universitaria, Col. San Manuel, Puebla, Puebla (Mexico)
The search of more specific and efficient photosensitizer in low oxygen tensions is a need in the Photodynamic Therapy (PDT). Phthalocyanines have demonstrated to have the above mentioned activity. The aim of this work was to determine the efficiency of PDT using two phthalocyanines synthesized in Mexico to eliminate melanoma cells. B16F0 melanoma mouse cells were exposed to concentrations from 8.95x10{sup -5} to 0.733 m/mL of F16VoPc and F16NbPcC13 during 24h, afterwards cellular mortality was measured. One kinetic was realized to determine the intracellular incorporation of phthalocyanines by confocal microscopy at 1, 2, 4, 8, 16 and 24 h of exposition. The PDT was applied exposing the cells to innocuous concentration (that does not provoke cellular death with out irradiation) and irradiating with an argon laser at 100 J/cm{sup 2}. For each phthalocyanine a control group was used; one group was not treated neither with light nor with phthalocyanine, the other group it was only irradiated. 24 h after treatment the citotoxicity was measured by Alamar blue assay. The innocuous concentration found for the phthalocyanines F16VoPc and F16NbPcC13 were 4.58x10-2 and 2.29xl0{sup -2} mg/mL, respectively. The time of maximum intracellular accumulation for both phthalocyanines was 24 h. Only the F16VoPc had anticancerous activity and induced 31.7% of cellular death. The PDT might offer a potential alternative to the treatment of this cancer when is used the phthalocyanine F16VoPc.
- OSTI ID:
- 21149288
- Journal Information:
- AIP Conference Proceedings, Vol. 1032, Issue 1; Conference: 10. Mexican symposium on medical physics, Mexico City (Mexico), 17-19 Mar 2008; Other Information: DOI: 10.1063/1.2979297; (c) 2008 American Institute of Physics; Country of input: International Atomic Energy Agency (IAEA); ISSN 0094-243X
- Country of Publication:
- United States
- Language:
- English
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