Antimicrobial activity of {gamma}-thionin-like soybean SE60 in E. coli and tobacco plants

Choi, Yeonhee; Choi, Yang Do

doi:10.1016/j.bbrc.2008.08.001

Title: Antimicrobial activity of {gamma}-thionin-like soybean SE60 in E. coli and tobacco plants

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Abstract

The SE60, a low molecular weight, sulfur-rich protein in soybean, is known to be homologous to wheat {gamma}-purothionin. To elucidate the functional role of SE60, we expressed SE60 cDNA in Escherichia coli and in tobacco plants. A single protein band was detected by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) after anti-FLAG affinity purification of the protein from transformed E. coli. While the control E. coli cells harboring pFLAG-1 showed standard growth with Isopropyl {beta}-D-1-thiogalactopyranoside (IPTG) induction, E. coli cells expressing the SE60 fusion protein did not grow at all, suggesting that SE60 has toxic effects on E. coli growth. Genomic integration and the expression of transgene in the transgenic tobacco plants were confirmed by Southern and Northern blot analysis, respectively. The transgenic plants demonstrated enhanced resistance against the pathogen Pseudomonas syringae. Taken together, these results strongly suggest that SE60 has antimicrobial activity and play a role in the defense mechanism in soybean plants.

Authors:

Choi, Yeonhee ^[1]; Choi, Yang Do ^[2]

School of Biological Sciences, Seoul National University, Seoul 151-747 (Korea, Republic of)
Department of Agricultural Biotechnology and Center for Agricultural Biomaterials, Seoul National University, Seoul 151-921 (Korea, Republic of)

Publication Date:: Fri Oct 17 00:00:00 EDT 2008

OSTI Identifier:: 21143903

Resource Type:: Journal Article

Journal Name:: Biochemical and Biophysical Research Communications

Additional Journal Information:: Journal Volume: 375; Journal Issue: 2; Other Information: DOI: 10.1016/j.bbrc.2008.08.001; PII: S0006-291X(08)01521-0; Copyright (c) 2008 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X

Country of Publication:: United States

Language:: English

Subject:: 60 APPLIED LIFE SCIENCES; ELECTROPHORESIS; ESCHERICHIA COLI; GELS; GLYCINE HISPIDA; MOLECULAR WEIGHT; NICOTIANA; PATHOGENS; PROTEINS; PSEUDOMONAS; SOYBEANS; TOXICITY; TRANSGENIC PLANTS; WHEAT

Citation Formats


                    Choi, Yeonhee, Choi, Yang Do, and Lee, Jong Seob. Antimicrobial activity of {gamma}-thionin-like soybean SE60 in E. coli and tobacco plants.  United States: N. p., 2008. 
        Web.  doi:10.1016/j.bbrc.2008.08.001.

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                    Choi, Yeonhee, Choi, Yang Do, & Lee, Jong Seob. Antimicrobial activity of {gamma}-thionin-like soybean SE60 in E. coli and tobacco plants.  United States.  https://doi.org/10.1016/j.bbrc.2008.08.001

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                    Choi, Yeonhee, Choi, Yang Do, and Lee, Jong Seob. 2008.  
        "Antimicrobial activity of {gamma}-thionin-like soybean SE60 in E. coli and tobacco plants".  United States.  https://doi.org/10.1016/j.bbrc.2008.08.001.

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@article{osti_21143903,

  title        = {Antimicrobial activity of {gamma}-thionin-like soybean SE60 in E. coli and tobacco plants},

  author       = {Choi, Yeonhee and Choi, Yang Do and Lee, Jong Seob},

  abstractNote = {The SE60, a low molecular weight, sulfur-rich protein in soybean, is known to be homologous to wheat {gamma}-purothionin. To elucidate the functional role of SE60, we expressed SE60 cDNA in Escherichia coli and in tobacco plants. A single protein band was detected by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) after anti-FLAG affinity purification of the protein from transformed E. coli. While the control E. coli cells harboring pFLAG-1 showed standard growth with Isopropyl {beta}-D-1-thiogalactopyranoside (IPTG) induction, E. coli cells expressing the SE60 fusion protein did not grow at all, suggesting that SE60 has toxic effects on E. coli growth. Genomic integration and the expression of transgene in the transgenic tobacco plants were confirmed by Southern and Northern blot analysis, respectively. The transgenic plants demonstrated enhanced resistance against the pathogen Pseudomonas syringae. Taken together, these results strongly suggest that SE60 has antimicrobial activity and play a role in the defense mechanism in soybean plants.},

  doi          = {10.1016/j.bbrc.2008.08.001},

  url          = {https://www.osti.gov/biblio/21143903},
  journal      = {Biochemical and Biophysical Research Communications},

  issn         = {0006-291X},

  number       = 2,

  volume       = 375,

  place        = {United States},

  year         = {Fri Oct 17 00:00:00 EDT 2008},

  month        = {Fri Oct 17 00:00:00 EDT 2008}

}

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