Essential roles of caspases and their upstream regulators in rotenone-induced apoptosis
- Graduate Institute of Veterinary Medicine, College of Biological Resources and Agriculture, National Taiwan University, Taipei, Taiwan (China)
- Department of Internal Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan (China)
- Graduate Institute of Oral Biology, College of Medicine, National Taiwan University, Taipei, Taiwan (China)
- Genomics Research Center, Academia Sinica, Taipei, Taiwan (China)
In the present study, we examined whether caspases and their upstream regulators are involved in rotenone-induced cytotoxicity. Rotenone significantly inhibited the proliferation of oral cancer cell lines in a dose-dependent manner compared to normal oral mucosal fibroblasts. Flow cytometric analysis of DNA content showed that rotenone treatment induced apoptosis following G2/M arrest. Western blotting showed activation of both the caspase-8 and caspase-9 pathways, which differed from previous studies conducted in other cell types. Furthermore, p53 protein and its downstream pro-apoptotic target, Bax, were induced in SAS cells after treatment with rotenone. Rotenone-induced apoptosis was inhibited by antioxidants (glutathione, N-acetylcysteine, and tiron). In conclusion, our results demonstrate significant involvement of caspases and their upstream regulators in rotenone-induced cytotoxicity.
- OSTI ID:
- 21143720
- Journal Information:
- Biochemical and Biophysical Research Communications, Vol. 371, Issue 1; Other Information: DOI: 10.1016/j.bbrc.2008.03.149; PII: S0006-291X(08)00615-3; Copyright (c) 2008 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
- Country of Publication:
- United States
- Language:
- English
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