Profiling TRA-1-81 antigen distribution on a human embryonic stem cell
Abstract
Human embryonic stem (hES) cells hold great promise in regenerative medicine. Although hES cells have unlimited self-renewal potential, they tend to differentiate spontaneously in culture. TRA-1-81 is a biomarker of undifferentiated hES cells. Quantitative characterization of TRA-1-81 expression level in a single cell helps capture the 'turn-on' signal and understand the mechanism of early differentiation. Here, we report on our examination of TRA-1-81 distribution and association on a hES cell membrane using an atomic force microscope (AFM). Our results suggest that aggregated distribution of TRA-1-81 antigen is characteristic for undifferentiated hES cells. We also evaluated the TRA-1-81 expression level at {approx}17,800 epitopes and {approx}700 epitopes per cell on an undifferentiated cell and a spontaneously differentiated cell, respectively. The method in this study can be adapted in examining other surface proteins on various cell types, thus providing a general tool for investigating protein distribution and association at the single cell level.
- Authors:
-
- Department of Biological, Chemical and Physical Sciences, Illinois Institute of Technology, 3101 S. Dearborn Street, Life Sciences Building, Chicago, IL 60616 (United States)
- Argonne National Laboratory, Biosciences Division, Lemont, IL 60439 (United States)
- Publication Date:
- OSTI Identifier:
- 21143658
- Resource Type:
- Journal Article
- Journal Name:
- Biochemical and Biophysical Research Communications
- Additional Journal Information:
- Journal Volume: 369; Journal Issue: 2; Other Information: DOI: 10.1016/j.bbrc.2008.02.102; PII: S0006-291X(08)00364-1; Copyright (c) 2008 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 60 APPLIED LIFE SCIENCES; ANTIGENS; ATOMIC FORCE MICROSCOPY; BIOLOGICAL MARKERS; CELL MEMBRANES; DRUGS; PROTEINS; STEM CELLS
Citation Formats
Dengli, Qiu, Jialing, Xiang, Zhaoxia, Li, Krishnamoorthy, Aparna, Liaohai, Chen, and Wang Rong. Profiling TRA-1-81 antigen distribution on a human embryonic stem cell. United States: N. p., 2008.
Web.
Dengli, Qiu, Jialing, Xiang, Zhaoxia, Li, Krishnamoorthy, Aparna, Liaohai, Chen, & Wang Rong. Profiling TRA-1-81 antigen distribution on a human embryonic stem cell. United States.
Dengli, Qiu, Jialing, Xiang, Zhaoxia, Li, Krishnamoorthy, Aparna, Liaohai, Chen, and Wang Rong. 2008.
"Profiling TRA-1-81 antigen distribution on a human embryonic stem cell". United States.
@article{osti_21143658,
title = {Profiling TRA-1-81 antigen distribution on a human embryonic stem cell},
author = {Dengli, Qiu and Jialing, Xiang and Zhaoxia, Li and Krishnamoorthy, Aparna and Liaohai, Chen and Wang Rong},
abstractNote = {Human embryonic stem (hES) cells hold great promise in regenerative medicine. Although hES cells have unlimited self-renewal potential, they tend to differentiate spontaneously in culture. TRA-1-81 is a biomarker of undifferentiated hES cells. Quantitative characterization of TRA-1-81 expression level in a single cell helps capture the 'turn-on' signal and understand the mechanism of early differentiation. Here, we report on our examination of TRA-1-81 distribution and association on a hES cell membrane using an atomic force microscope (AFM). Our results suggest that aggregated distribution of TRA-1-81 antigen is characteristic for undifferentiated hES cells. We also evaluated the TRA-1-81 expression level at {approx}17,800 epitopes and {approx}700 epitopes per cell on an undifferentiated cell and a spontaneously differentiated cell, respectively. The method in this study can be adapted in examining other surface proteins on various cell types, thus providing a general tool for investigating protein distribution and association at the single cell level.},
doi = {},
url = {https://www.osti.gov/biblio/21143658},
journal = {Biochemical and Biophysical Research Communications},
issn = {0006-291X},
number = 2,
volume = 369,
place = {United States},
year = {Fri May 02 00:00:00 EDT 2008},
month = {Fri May 02 00:00:00 EDT 2008}
}