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Title: Profiling TRA-1-81 antigen distribution on a human embryonic stem cell

Abstract

Human embryonic stem (hES) cells hold great promise in regenerative medicine. Although hES cells have unlimited self-renewal potential, they tend to differentiate spontaneously in culture. TRA-1-81 is a biomarker of undifferentiated hES cells. Quantitative characterization of TRA-1-81 expression level in a single cell helps capture the 'turn-on' signal and understand the mechanism of early differentiation. Here, we report on our examination of TRA-1-81 distribution and association on a hES cell membrane using an atomic force microscope (AFM). Our results suggest that aggregated distribution of TRA-1-81 antigen is characteristic for undifferentiated hES cells. We also evaluated the TRA-1-81 expression level at {approx}17,800 epitopes and {approx}700 epitopes per cell on an undifferentiated cell and a spontaneously differentiated cell, respectively. The method in this study can be adapted in examining other surface proteins on various cell types, thus providing a general tool for investigating protein distribution and association at the single cell level.

Authors:
; ; ;  [1];  [2]
  1. Department of Biological, Chemical and Physical Sciences, Illinois Institute of Technology, 3101 S. Dearborn Street, Life Sciences Building, Chicago, IL 60616 (United States)
  2. Argonne National Laboratory, Biosciences Division, Lemont, IL 60439 (United States)
Publication Date:
OSTI Identifier:
21143658
Resource Type:
Journal Article
Journal Name:
Biochemical and Biophysical Research Communications
Additional Journal Information:
Journal Volume: 369; Journal Issue: 2; Other Information: DOI: 10.1016/j.bbrc.2008.02.102; PII: S0006-291X(08)00364-1; Copyright (c) 2008 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ANTIGENS; ATOMIC FORCE MICROSCOPY; BIOLOGICAL MARKERS; CELL MEMBRANES; DRUGS; PROTEINS; STEM CELLS

Citation Formats

Dengli, Qiu, Jialing, Xiang, Zhaoxia, Li, Krishnamoorthy, Aparna, Liaohai, Chen, and Wang Rong. Profiling TRA-1-81 antigen distribution on a human embryonic stem cell. United States: N. p., 2008. Web.
Dengli, Qiu, Jialing, Xiang, Zhaoxia, Li, Krishnamoorthy, Aparna, Liaohai, Chen, & Wang Rong. Profiling TRA-1-81 antigen distribution on a human embryonic stem cell. United States.
Dengli, Qiu, Jialing, Xiang, Zhaoxia, Li, Krishnamoorthy, Aparna, Liaohai, Chen, and Wang Rong. 2008. "Profiling TRA-1-81 antigen distribution on a human embryonic stem cell". United States.
@article{osti_21143658,
title = {Profiling TRA-1-81 antigen distribution on a human embryonic stem cell},
author = {Dengli, Qiu and Jialing, Xiang and Zhaoxia, Li and Krishnamoorthy, Aparna and Liaohai, Chen and Wang Rong},
abstractNote = {Human embryonic stem (hES) cells hold great promise in regenerative medicine. Although hES cells have unlimited self-renewal potential, they tend to differentiate spontaneously in culture. TRA-1-81 is a biomarker of undifferentiated hES cells. Quantitative characterization of TRA-1-81 expression level in a single cell helps capture the 'turn-on' signal and understand the mechanism of early differentiation. Here, we report on our examination of TRA-1-81 distribution and association on a hES cell membrane using an atomic force microscope (AFM). Our results suggest that aggregated distribution of TRA-1-81 antigen is characteristic for undifferentiated hES cells. We also evaluated the TRA-1-81 expression level at {approx}17,800 epitopes and {approx}700 epitopes per cell on an undifferentiated cell and a spontaneously differentiated cell, respectively. The method in this study can be adapted in examining other surface proteins on various cell types, thus providing a general tool for investigating protein distribution and association at the single cell level.},
doi = {},
url = {https://www.osti.gov/biblio/21143658}, journal = {Biochemical and Biophysical Research Communications},
issn = {0006-291X},
number = 2,
volume = 369,
place = {United States},
year = {Fri May 02 00:00:00 EDT 2008},
month = {Fri May 02 00:00:00 EDT 2008}
}